Thousands of people beat cancer every year. Doing so is easier when cancer is diagnosed at an early stage as treatment is often simpler and more likely to be effective. Cancer cells starts out as normal body cells, but they begin to grow out of control because of an abnormal gene expression. The immune system plays a major role in limiting the development of these abnormalities. It elicits a de...

Most patients with chronic immune thrombocytopenic purpura (ITP) have autoantibodies directed against the glycoprotein (GP) IIb/IIIa complex. We have used a filamentous phage library that displays random linear hexapeptides to identify peptide sequences recognized by these autoantibodies. Plasma antibody eluates from two patients were used to select for phage displaying autoantibody-reactive pe...

We have used the Src homology 3 (SH3) domain to screen two phage-displayed random peptide libraries, each containing 2 x 10(8) unique members, and have identified a series of high affinity peptide ligands. The peptides possess similar proline-rich regions, which yield a consensus Src SH3-binding motif of RPLPPLP. We have confirmed this motif by screening a phage-displayed peptide library biased...

mAb73, a monoclonal antibody against adenoviruses type 2 and 5 E1A, recognises an epitope within the C-terminal part of this protein. To identify the epitope we used random peptide libraries expressed on the surface of filamentous phages (Fd, M13). We found a consensus sequence homologous to the nuclear transport signal KRPRP at the C-terminus of Ad2 and Ad5 E1A. An E1A mutant deleted for these...

We are developing a new method for protein structure determination that overcomes limitations in traditional methods, such as x-ray crystallography or nuclear magnetic resonance, and requires about a thousand fold less protein. The method, called Antibody Imprinting, uses antibodies against target proteins and random peptide probe libraries to map the epitopes of antibody binding sites on targe...

Peptide libraries have proven to be useful in applications such as substrate profiling, drug candidate screening and identifying protein-protein interaction partners. However, issues of fidelity, peptide length and purity have been encountered when peptide libraries are chemically synthesized. Biochemically produced libraries, on the other hand, circumvent many of these issues due to the fideli...