Identified germplasm is an important component for efficient and effective management of plant genetic resources. Traditionally, cultivars or species identification has relied on morphological characters like growth habit or floral morphology like flower colour and other characteristics of the plant. Studies were undertaken for identification and analysis of genetic variation within 34 rose cul...

Identified germplasm is an important component for efficient and effective management of plant genetic resources. Traditionally, plant identification has relied on morphological characters like growth habit, floral morphology like flower colour and other characteristics of the plant. Studies were undertaken for identification and genetic variation within 15 clones of Tinospora cordifolia throug...

Random amplified polymorphic DNA (RAPD) analysis was employed to estimate genetic diversity/genetic similarity among nine wheat (Triticum aestivum L.) genotypes. Out of 25 random 10-mer primers surveyed, 18 primers detected polymorphism among all the wheat genotypes, while 7 primers produced monomorphic pattern. A total of 175 bands were amplified, 60.57% of which were polymorphic. Genetic simi...

The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides ...

Urtica dioica is an ethnobotanically and medicinally important Complementary and Alternative Medicine (CAM) plant worldwide and in Turkey; 90 % of herbal CAM applications depend on it in Turkey. It has a wide range of habitats in nearly all continents. It is found in all three phytogeographical regions in Turkey (Euro-Siberian, Irano-Turanian, Mediterranean) with high adaptivity to heterogeneou...

In vitro random mutagenesis is a powerful tool for altering properties of enzymes. We describe here a novel random mutagenesis method using rolling circle amplification, named error-prone RCA. This method consists of only one DNA amplification step followed by transformation of the host strain, without treatment with any restriction enzymes or DNA ligases, and results in a randomly mutated plas...

Escherichia coli O157:H7, an emerging cause of food-borne disease, has now been reported from several countries worlwide. Cattle and sheep are known as a principle reservoir of this agent. It is also prevalent in gastrointestinal tract of other animals like pigs, dogs, cats and birds. This agent can be transmitted to humans by food and water and even person to person contact. The random amplifi...

Random ampli®ed polymorphic DNAs (RAPDs) were used to discriminate among 25 Feijoa sellowiana cultivars and accessions. Fifty ten-mer oligonucleotide primers were tested on ®ve accessions. Twenty-two primers, showing clear polymorphic patterns, were chosen to amplify the DNAs of all 25 genotypes; each ampli®cation was repeated three times separately. Only 11 primers were ®nally analysed, yieldi...

The objective of this study was to distinguish between males and females of jojoba (Simmondisia chinensis) using Random Amplified Polymorphic DNA (RAPD) markers in order to detect any DNA variation. A callus was produced from both male and female jojoba leaves, using MS medium with BA 0.5 and 2 to 4, D 2.5 mg/l respectively. Genomic DNA of jojoba plant was extracted using CTAB method. The conce...

Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA polymerase with the objective of determining t...