reactive oxygen species (ros) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. curcumin is known as an effective antioxidant against oxidative stress. the aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and dna integrity of rat spermatozoa during semen freeze-tha...

DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from eukaryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)...

SUMMARY Alkaline sucrose gradients were used to study the induc tion of single-strand breaks in and repair of rat liver DNA in vivo. Liver DNA was labeled during regeneration after partial hepatectomy. DNA was isolated from the organ in such a way as to minimize the induction of breaks due to handlingor enzymaticactivity.Differentmethodsof prepar ing high-molecular-weight liver DNA were compare...

was incorporation of the radioactive label in the DNA extracted from various organs. In the rat, concentration of radioactivity in the DNA of each organ was appreciably higher at 24 hours than at 1 hour after injection and was increased at the latter interval by pretreatment with 5-fluorodeoxyuridine. Incorporation of ICdR into DNA of the rat was quantitatively less than that previ ously report...

The involvement of serotonin and its receptor subtype in the induction of hepatocyte DNA synthesis was investigated in primary cultures of adult rat hepatocytes. Serotonin caused a dose-dependent increase in DNA synthesis in primary cultures of rat hepatocytes in the presence of epidermal growth factor (EGF) and insulin, as measured by [3H]thymidine incorporation. The serotonin S2 receptor anta...

The effects of Kilham rat virus multiplication were studied in cultured rat embryo cells to examine the mechanisms by which virus infection might be related to developmental defects in rats and hamsters. The virus was found to inhibit motosis and deoxyribonucleic acid (DNA) synthesis within 2 to 10 hr after infection. However, total ribonucleic acid synthesis was relatively unaffected until abo...