نتایج جستجو برای: recombinant dna
تعداد نتایج: 597179 فیلتر نتایج به سال:
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...
With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
Background: Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures.Objectives: Molecular cl...
DNA–Protein cross-links (DPCs) are cytotoxic DNA lesions with a protein covalently bound to the DNA. Although much has been learned about formation, repair, and biological consequences of DPCs in nucleus, little is known regarding mitochondrial DPCs. This due part lack robust specific methods measure Herein, we reported an enzyme-linked immunosorbent assay (ELISA)-based method for detecting for...
Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women. Serological tests with sensitivities related to specific antigens are commonly used as routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
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