BIOPHYSICS AND COMPUTATIONAL BIOLOGY, ENGINEERING Correction for “Biomechanics of red blood cells in human spleen and consequences for physiology and disease,” by Igor V. Pivkin, Zhangli Peng, George E. Karniadakis, Pierre A. Buffet, Ming Dao, and Subra Suresh, which appeared in issue 28, July 12, 2016, of Proc Natl Acad Sci USA (113:7804–7809; first published June 27, 2016; 10.1073/pnas.160675...

Abstract The red blood cell (RBC) antigen Wr a is low-prevalence first described in 1953 by Holman and assigned to the Diego system 1995. Because of its low prevalence, usually absent on commercial screening RBCs antibody identification panels. When Wr(a+) are available, corresponding antibody, anti-Wr , often found sera from healthy individuals, patients, pregnant women. Anti-Wr can cause both...

OBJECTIVE Transfusion of aged blood has been associated with increased morbidity and mortality in critically ill patients. During storage, erythrocytes release increasing numbers of microvesicles (red blood cell-derived microvesicles [RBC-MV]). We hypothesized that RBC-MV mediate some of the deleterious effects of aged blood transfusions. APPROACH AND RESULTS We established a murine transfusi...

BACKGROUND Decisions for when to select, and when to discontinue, antigen-negative blood in hematopoietic progenitor cell transplantation (HPCT) recipients with red blood cell (RBC) antibodies can be confusing. In HPCT performed for sickle cell anemia patients who require extremely rare antigen-negative blood, the balance of caution and practicality is further complicated. CASE REPORTS Four s...

A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our method combines a liquid flow cell, fluorescence microscopy, and an optical-trap to facilitate simple measurements of the shear modulus and buckling properties of single RBCs, under physiological conditions. The efficacy of the method is illustrated ...

Introduction In brain-injured patients, the appropriateness of restrictive red blood cell (RBC) transfusion strategies is uncertain given the brain’s vulnerability to hypoxemia. Current practice is based on small clinical trials and physiologic data from clinical and preclinical studies. Little is known regarding how preclinical data has informed RBC transfusion in this specific patient populat...

Tissue occlusion and the hyperaemic response upon reperfusion can be used as a tool to assess microvascular function in various vascular diseases. Currently, laser Doppler flowmetry (LDF) is applied most often to measure hyperaemic responses. In this study, we have applied tissue viability imaging (TiVi) and LDF to measure the change in red blood cell concentration and perfusion in the skin aft...

The performance of the Sorin Xtra® Autotransfusion System (ATS) was studied in 62 patients undergoing coronary artery bypass grafting. Blood was collected intraoperatively and washed using three different wash sets in 4 groups. Both collected and washed blood were analysed for hemoglobin levels and hematocrit, concentrations of proteins, albumin, heparin and plasma free hemoglobin (PFH) were de...

Background and Aims: Iron deficiency anemia (IDA) and beta thalassemia minor (BTM) are the most common hypochromic microcytic anemias, and it is regarded important to differentiate between them. several formulae can be taken into consideration based on red blood cell (RBC) parameters in order to distinguish between these two disorders. Hence, the present study intended to evaluate the sensitivi...