The method for the estimation of oxidized and reduced glutathione (GSSG and GSH) based on the glyoxalase reaction (Woodward, 1935; Martin & Mcllwain, 1959) is the only method available with a high degree of specificity. The present paper suggests another specific method based on thioldisulphide exchange reactions. Pihl, Eldjarn & Bremer (1957) have shown that exchange reactions between GSH and ...

A proximo-distal gradient of reduced glutathione (GSH), a non enzymatic antioxidant was observed in the original tails of the lizard, H. leschenaultii. In the regenerating tails, a gradual increase in the level of GSH was noted with tail elongation. In the newly regenerated tails also the level of GSH remained higher in the proximal part than the corresponding distal parts.

This studies whether the water extract of Osmanthus fragrans can prevent noise-induced hearing loss (NIHL) after exposure to a noisy environment. Twenty-four healthy factory workers in southern Taiwan were recruited. The hearing threshold was determined before and after each dosing period. Measurements of oxygen radical absorbance capacity (ORAC), glutathione (GSH), oxidized glutathione disulfi...

The role of exogenous spermidine (Spd) in alleviating low temperature (LT) stress in mung bean (Vigna radiata L. cv. BARI Mung-3) seedlings has been investigated. Low temperature stress modulated the non-enzymatic and enzymatic components of ascorbate-glutathione (AsA-GSH) cycle, increased H₂O₂ content and lipid peroxidation, which indicate oxidative damage of seedlings. Low temperature reduced...

BACKGROUND Previous studies showed that supplementation of reduced form of glutathione (GSH, 500 mg/d) has a skin-lightening efficacy in humans. This study was designed to evaluate the influences of both GSH and oxidized form (GSSG), at doses lower than 500 mg/d, on improving skin properties. PATIENTS AND METHODS A randomized, double-blind, placebo-controlled, parallel, three-arm study was co...

Glutathione is a tripeptide involved in various aspects of plant metabolism. This study investigated the effects of the reduced form of glutathione (GSH) applied to specific organs (source leaves, sink leaves, and roots) on cadmium (Cd) distribution and behaviour in the roots of oilseed rape plants (Brassica napus) cultured hydroponically. The translocation ratio of Cd from roots to shoots was ...

We previously found that reduced glutathione (GSH) or a mixture of GSH/glutathione disulfide (GSSG) potentiated platelet aggregation. We here report that GSSG, when added to platelets alone, also potentiates platelet aggregation. Most of the GSSG was converted to GSH by a flavoprotein-dependent platelet surface mechanism. This provided an appropriate redox potential for platelet activation. The...

homalium letestui pellegr (flacourtiaceae) is used traditionally by the ibibios of southern nigeria to treat stomach ulcer, malaria and other inflammatory diseases and yorubas of western nigeria as an antidote. the hepatoprotective effect of the stem extract (200-600 mg/kg) was evaluated by the assay of liver function parameters, namely total and direct bilirubin, serum protein and albumin, tot...

Glutathione (GSH) is the major scavenger of reactive oxygen species (ROS) inside cells. We used live confocal imaging in order to clarify the role of GSH in the biology of the organ of Corti, the sensory epithelium of the cochlea, before, during and after the onset of hearing and in ~1 year old mice. GSH content was measured using monochlorobimane (MCB), a non-fluorescent cell permeant bimane t...

Glutathione disappears as a soluble, nonprotein component after it has been added to serum, either in the reduced (GSH) or oxidized (GSSG) form. When GSH is added to serum it is rapidly oxidized to GSSG by some dialyzable component of serum. Glutathione is lost because of interaction of serum proteins with the GSSG formed in this manner or added directly. A similarly rapid loss of glutathione o...