We reported previously the finding by paper chromatography of reducing sugars in the urines of 24 out of 50 normal full-term babies (Haworth and McCredie, 1956). We suggested that the galactose which we found in the urines of 13 of the 50 babies might be related to the functional inefficiency of the liver that is so commonly a feature of the newborn. If this were so it might be expected that pr...

M. (1992) Purification and characterization of human salivarygland prokallikrein from recombinant baculovirus-infected insect cells. Eur. J. Biochem. 206, 225–233. 6. Takada, Y., Skidgel, R. A., and Erdös, E. G. (1985) Purification of human urinary prokallikrein. Biochem. J. 232, 851–858. 7. Noda, Y., Takada, Y., and Erdös, E. G. (1985) Activation of human and rabbit prokallikrein by serine and...

An attempt was made to localize a number of O-methyl sugars in lipopolysaccharides and antigenic polysaccharides isolated from photosynthetic bacteria and from cyano-bacteria. Methylation analysis with [2H3]methyl iodide as methylating agent was the method of choice. One has to differentiate between (lipo-)polysaccharides having only trace amounts (less than 1% of polymer dry weight) of O-methy...

The accumulation of reducing sugars in potato tubers exposed to low temperatures occurs with concomitant formation of the enzyme invertase. During the initial period of cold treatment when reducing sugars increase rapidly, invertase formation proceeds until the level of enzyme exceeds that of an endogenous macromolecular invertase inhibitor, resulting in a basal invertase activity. As the rate ...

A microtiter plate adaptation of the well-known dinitrosalicylic acid (DNS) colorimetric method, for measurement of reducing sugars, is described. This method allows a substantial volume reduction of the reagents and a rapid analysis of a large number of samples, having an economic cost benefit and a positive impact on the environment. Therefore, it is well suited as an high-throughput techniqu...

Coastal Bermuda grass was pretreated with NaOH at concentrations from 0.5% to 3% (w/v) for a residence time from 15 to 90min at 121 degrees C. The pretreatments were evaluated based on total lignin removal and production of total reducing sugars, glucose and xylose from enzymatic hydrolysis of the pretreated biomass. Up to 86% lignin removal was observed. The optimal NaOH pretreatment condition...

The MUNSON-WALKER method (3) with the modification of BERTRAND (1) has long been used for the determination of reducing sugars in plant extracts. The large amiiounts of solutions required by this method, however, make necessary the frequent preparation and standardization of solutions, which is inconvenient when large numbers of determinations are being made over an extended period of time. For...

To clarify the effects of storage temperature on potato components and acrylamide in chips, tubers from five cultivars were stored at various temperatures (2, 6, 8, 10, and 18 degrees C) for 18 weeks, and the contents of sugars, free amino acids in tubers, and acrylamide in chips after frying were analyzed. At temperatures lower than 8 degrees C, the contents of reducing sugars increased marked...