The purification and characterization of a new restriction endonuclease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. The enzyme preparation obtained is suitable for restriction mapping an ligation.

We present unzipping force analysis of protein association (UFAPA) as a novel and versatile method for detection of the position and dynamic nature of protein-DNA interactions. A single DNA double helix was unzipped in the presence of DNA-binding proteins using a feedback-enhanced optical trap. When the unzipping fork in a DNA reached a bound protein molecule we observed a dramatic increase in ...

The genomes of leptospiral field isolates belonging to serogroup Pomona were analyzed and compared with those of type strains by cleavage with restriction endonucleases. This new classification method shows differences among these organisms not indicated by the conventional serological typing method. No differences were observed among isolates from the United States, Canada, and New Zealand. Al...

Incomplete particles arising during productive growth of adenovirus were separated from infectious particles by density gradient centrifugation. The DNA contained in particles of low density was characterized by restriction enzyme analysis and by electron microscopy and heteroduplexing techniques. The DNA is heterogeneous in length, ranging in size from 15% of the normal genome to full length. ...

The adenovirus type 7 (Ad7) isolates from the 1995 nationwide outbreak in Japan were genetically and seroepidemiologically analyzed in comparison with Japanese Ad7 strains isolated before 1995 to determine their genome type and to speculate on their origin and causative factors of the outbreak. Twenty-six Ad7 isolates from the outbreak were identified by restriction enzyme analysis as the Ad7d2...

Methylation of Marek's disease virus serotype 1 (MDV1) DNA in both productively and latently infected cells was examined by restriction endonuclease analyses with the isoschizomeric pair HpaII and MspI. The latent MDV1 DNA in T-lymphoblastoid cell lines established from chicken T-lymphomas was considerably methylated, whereas methylation of the virus DNA sequences was not detected in productive...

The relative binding affinities of Mnt protein from bacteriophage P22 are determined for each possible base pair at position 17 of the operator. These are determined from the partitioning of randomized operators into bound and unbound fractions; quantitation is provided by restriction enzyme analysis. Mnt protein is found to have an unusual specificity at this position: a C.G base pair (the wil...

In the title mol-ecule, C(10)H(14)N(4)S, the thio-rea plane and benzene ring form a dihedral angle of 16.0 (3) Å. In the crystal structure, inter-molecular N-H⋯S hydrogen bonds link the mol-ecules into ribbons extended in the [100] direction; these incorporate inversion dimers.

A method has been devised whereby certain complete restriction digestions can have short overlaps of unique sequence incorporated into the fragments during cloning. Thus one can identify when the DNA fragments are contiguous. Here, this technique is used to produce a contig for a 2.5 kb fragment of genomic DNA. This is a simple approach to ordering digestion fragments without necessarily perfor...

In Clark v. Arizona, the U.S. Supreme Court was faced with two main questions: Does Arizona's insanity defense statute, with its abridged M'Naughten standard, violate the Fourteenth Amendment? And does Arizona case law, with its complete prohibition on the use of mental disease or defect evidence to combat required mens rea elements of a crime, violate due process? In a six-three decision, the ...