Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing ...

This article refers to viewing the role of molecular markers during analyzing genome plants and their importance in plant biotechnology. In recent years, we observed techniques programs for improving breeding preserving genetic resources has been observed, biochemical indicators which represent basic material through determining diversity between genotypes it is never affected by external surro...

Rust, incited by Uromyces appendiculatus^ is a major disease in common bean {Phaseolus vulgaris L,). Plant growth habit is also an important trait. Indeterminate growth habit was first reported to be controlled by a single dominant gene (Fin) (Lamprecht, 1935). Molecular markers such as isozymes, RFLP, and RAPD, have been used to tag genes for many traits, particularly disease resistance in sev...

We have developed a new technique for the physical mapping of barley chromosomes using microdissected translocation chromosomes for PCR with sequence-tagged site primers derived from >300 genetically mapped RFLP probes. The positions of 240 translocation breakpoints were integrated as physical landmarks into linkage maps of the seven barley chromosomes. This strategy proved to be highly efficie...

BACKGROUND & OBJECTIVES The search for effective polymorphic markers in Plasmodium vivax is highly demanding to understand its transmission in a population. Due to the limited knowledge existing for P. vivax, the search for polymorphic markers for population studies is ongoing. The MSP gene family of Plasmodia has been linked with immune evasion. To study the circulating parasite population P. ...

Primula species provide possibly the best known examples of heteromorphic flower development and this breeding system has attracted considerable attention, including that of Charles Darwin. However, despite considerable recent advances in molecular genetics, nothing is known about the molecular basis of floral heteromorphy. The first molecular marker for the Primula S-locus is reported here. Th...

Little information is available on genetic variation within and between populations of pest cockroaches. In this study, intraspecific Hind III polymorphism was investigated in the German cockroach, Blattella germanica (Linnaeus) (Dictyoptera, Blattaria: Blattellidae), using restriction fragment length polymorphisms (RFLP) of the non-transcribed spacer (NTS) region of ribosomal DNA (rDNA). Indiv...

this study determines the value of linkage analysis using six rflp markers for carrier detection and prenatal diagnosis in familial dmd/bmd cases and their family members for the first time in the iranian population. we studied the dystrophin gene in 33 unrelated patients with clinical diagnosis of dmd or bmd. subsequently, we determined the rate of heterozygosity for six intragenic rflp marker...

characteristics (Lambert and Tyler, 1999). The lack of development of superior SIR cultivars may be due to There has been limited success over the past 30 yr in the developthe quantitative nature of resistance and to the retention ment of superior soybean cultivars [Glycine max (L.) Merr] with of undesirable PI donor alleles affecting any number insect resistance. Success may be hampered by the...

binding site of the AFP gene promoter in one (2 ). AFP is a widely used tumor marker. Although HPAFP is a rare disorder, it needs to be considered and differentiated from tumors to avoid inappropriate explorations and treatment decisions. We present a simple test based on restriction fragment length polymorphism (RFLP) analysis for screening of HPAFP mutations. This test can be used in patients...