نتایج جستجو برای: rflp method genetic polymorphism
تعداد نتایج: 2232291 فیلتر نتایج به سال:
Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed o...
The study aimed to determine the genotypic distribution of c.328 G>A (rs110652478) polymorphism in bovine fatty acid-binding protein 4 (FABP4) gene and its effect on slaughter weight Aberdeen Angus Hereford bulls imported into Turkey. A total randomly selected 95 were used this study. Bulls housed for fattening semi-open pens approximately nine months with same management procedures. Genotyp...
OBJECTIVE To describe 12 yr experience of molecular genetic diagnosis of Spinal Muscular Atrophy (SMA) in 460 cases of Turkish patients. MATERIALS & METHODS A retrospective analysis was performed on data from 460 cases, referred to Medical Genetics Laboratory, Ege University's Hospital, Izmir, Turkey, prediagnosed as SMA or with family history of SMA between 2003 and 2014. The PCR-restriction...
Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the gene...
We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of...
BACKGROUND Genetic polymorphisms in the human MDM2 gene are suggested to be a tumor susceptibility marker and a prognostic factor for cancer. It has been reported that a single nucleotide polymorphism (SNP) c.309T>G in the MDM2 gene attenuates the tumor suppressor activity of p53 and accelerates tumor formation in humans. METHODOLOGY In this study, to detect the SNP c.309T>G in the MDM2 gene,...
Hepatitis B is one of the most common infectious diseases in the world, and 350 million people have been estimated to be chronic hepatitis B virus carriers world-wide. Hepatitis B virus (HBV) has been classified into 8 genotypes (A-H) based on an intergroup divergence of 8% or more in the complete nucleotide sequence. Different genotypes of the hepatitis B virus may influence the clinical outco...
Objective: Obsessive-compulsive disorder (OCD) is a frequent neuropsychiatric disorder, in which genetic factors play important causative roles. We investigated the roles of the (-1019 C/G) promoter region polymorphism of 5-HTR1A and the G861C coding region polymorphism of 5-HTR1B genes in susceptibility to OCD in a Turkish population. Methods: Two single nucleotide polymorphisms, 5-HTR1A (rs62...
This investigation was conducted to identify polymorphism of the leptin gene in Holstein cows, Mazandarani native cattle and buffaloes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequencies of A and B alleles were 0.4 and 0. 6 in Holstein cows, 0.56 and 0.44 in Mazandarani native cattle, 0.61 and 0.35 in buffaloes, respectively. The heterozygote genoty...
Garlic belongs to the Allium genus, which includes more than 750 species divided into 60 taxonomic groups. It is cultivated in many countries throughout world for bulb and used as a spice functional food. The plant vegetatively propagates. This review will focus on origins, biology, analysis of genetic diversity, pharmacological properties garlic. appears from this synthesis that sativum derive...
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