Due to its high sensitivity and reproducibility, quantitative real-time PCR (qPCR) is practiced as a useful research tool for targeted gene expression analysis. For qPCR operations, the normalization with suitable reference genes (RGs) is a crucial step that eventually determines the reliability of the obtained results. Although pepper is considered an ideal model plant for the study of non-cli...

Elevating serotonin (5-HT) levels with selective serotonin reuptake inhibitors (SSRIs) is the most widely used treatment for depression. However, current therapies are ineffective, have delayed benefit, or cause side effects in many patients. Here, we define a mechanism downstream of 5-HT1A receptors that mediates antidepressant-like behavior and is profoundly and selectively enhanced by geneti...

Regulator of G-protein signaling (RGS) proteins classically function as negative modulators of G-protein-coupled receptor signaling. In vitro, RGS proteins have been shown to inhibit signaling by agonists at the μ-opioid receptor, including morphine. The goal of the present study was to evaluate the contribution of endogenous RGS proteins to the antinociceptive effects of morphine and other opi...

BACKGROUND RGS family members are GTPase-activating proteins for heterotrimeric Gq and Gi proteins. RGS genes are expressed in heart tissue and in cultured cardiomyocytes. There is evidence that altered RGS gene expression may contribute to the pathogenesis of cardiac hypertrophy and failure. METHODS AND RESULTS We investigated the ability of RGS proteins to block G-protein signaling in vivo ...

[1] Estimation under model uncertainty remains a practical concern in many scientific and engineering fields. A commonly encountered example in groundwater remediation is the contaminant source identification problem. Like many other inverse problems, contaminant source identification is inherently ill posed and is sensitive to both data and model uncertainties. Model uncertainties, which may b...

The regulator of G protein signaling (RGS) family of proteins serves critical roles in G protein-coupled receptor (GPCR) and heterotrimeric G protein signal transduction. RGS proteins are best understood as negative regulators of GPCR/G protein signaling. They achieve this by acting as GTPase activating proteins (GAPs) for Gα subunits and accelerating the turnoff of G protein signaling. Many RG...

Abstract In previous funding literature, ample attention has been devoted to the consequences of competition for project grants. These tend be fueled by status distinctions among grants, but scant directed toward how such are constructed. My aim with this paper is develop new knowledge about ways in which scientists ascribe meanings that construct Employing qualitative data and a Bourdieu-inspi...

Refuge Green Space (RGS) plays roles in both rapid evacuation and short-term resettlement response to sudden natural hazards, especially geological disasters. As a result, its evaluation location optimization are crucial for the development of sustainable cities. In this study, Three-Step Floating Catchment Area Method (3SFCA) Moran’s I index were used as measurement models. The capacity RGS ev...

Concerns over interactions between analgesics and experimental outcomes are a major reason for withholding opioids from rats undergoing surgical procedures. Only a fraction of morphine injected intravenously reaches receptors responsible for analgesia in the central nervous system. Intrathecal administration of morphine may represent a way to provide rats with analgesia while minimizing the amo...

Chronic exposure of cells to mu-opioid agonists leads to tolerance which can be measured by a reduced ability to activate signaling pathways in the cell. Cell signaling through inhibitory G proteins is negatively regulated by RGS (regulator of G protein signaling) proteins. Here we examine the hypothesis that the GTPase accelerating activity of RGS proteins, by altering the lifetime of Galpha a...