by a double displacement mechanism (1, 2). Thiosulfate and organic thiosulfonates will act as sulfur donors for the enzyme (3). Cyanide ion, SOS=, and the organic sulfinates will accept sulfur from the enzyme-sulfur compound (1, 4, 5). In this study, crystalline beef liver rhodanese has been found to catalyze the reduction of SSOp to HSand SOf. Several powerful reducing agents, such as NaBH4 an...

Chemical studies of rhodanese (thiosulfate : cyanide sulfurtransferase, EC 2.8.1.1) in this laboratory have prompted the development of a modified procedure for preparing the crystalline enzyme. The new method, which is based on the earlier procedure of S6rbo (1) as modified by Westley et al. (2-4), permits isolation in apparently monodisperse form of nearly 50% of the total rhodanese activity ...

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.

The activity of rhodanese, 3-mercaptopyruvate sulfurtransferase and gamma-cystathionase and the content of glutathione and sulfane sulfur compounds were determined in Rana temporaria brain in April. The high sulfane sulfur level observed in the spring seems to be associated with protection against cellular oxidative stress after the period of hibernation with its minimal oxidative metabolism.