The efficacy of neutral protease and combinations of neutral protease and highintensity ultrasound on rice starch isolation was investigated. Rice flour slurry (33%) was treated with neutral protease at 0.01, 0.03, or 0.05% (on flour basis) or combinations of 0.03% neutral protease and high-intensity ultrasound at 25, 50, or 75% amplitude for 15, 30, or 60 min concurrently or sequentially. The ...

Rice (Oryza sativa L) is the second largest important cultivated cereal. India is among major rice producing countries in world and Asia contributed 90 percent of the world cultivated area. India contributes more than 20% of paddy production in world with a figure of 152.6 MMT in 2012. Rice being invaluable alternative source of carbohydrate being easily digestible and has rare allergic reactio...

Starch is a biologically and commercially important polymer of glucose. Starch is organized into starch grains (SGs) inside amyloplasts. The SG size differs depending on the plant species and is one of the most important factors for industrial applications of starch. There is limited information on genetic factors regulating SG sizes. In this study, we report the rice (Oryza sativa) mutant subs...

A rice (Oryza sativa L.) genomic clone encoding the gene for a form of soluble starch synthase (SSS1) and its 5'- and 3'-flanking regions has been isolated and sequenced. The SSS1 gene contained 15 exons interrupted by 14 introns. The exon/intron organization of the SSS1 gene was divergent from that of the rice Waxy gene coding for granule-bound starch synthase, thus suggesting that the SSS1 an...

To elucidate the starch synthesis pathway and the role of this reserve in rice pollen, we characterized mutations in the plastidic phosphoglucomutase, OspPGM, and the plastidic large subunit of ADP-glucose (ADP-Glc) pyrophosphorylase, OsAGPL4 Both genes were up-regulated in maturing pollen, a stage when starch begins to accumulate. Progeny analysis of self-pollinated heterozygous lines carrying...

Starches from various botanical origins (rice, maize and potato starches) were cross-linked in the presence of epichlorohydrin (EPI), respectively. Then, the cross-linked starches were further oxidized with sodium hypochlorite (NaOCl, 2.5% w/w) to obtain oxidized cross-linked starches (OCS). The chemically modified methods on the physicochemical properties of different starches were investigate...

Native rice starch (NRS, amylose/28.9%) was gelatinized by improved extrusion cooking technology (IECT) and retrograded (RRS) after low temperature storage (4 °C). The retrogradation behaviour of RRS was changed to low retrogradation percentage and low retrogradation rate. The retrogradation resulted in a high compact morphology. The melt enthalpy change and percentage of retrogradation of RRS ...

Rice (Oryza sativa) endosperm is mainly occupied by homogeneous polygonal starch from inside to outside. However, morphologically different (heterogeneous) starches have been identified in some rice mutants. How these heterogeneous starches form remains unknown. A high-amylose rice line (TRS) generated through the antisense inhibition of starch branching synthase I (SBEI) and SBEIIb contains fo...

We investigated sugar uptake and transport in rice (Oryza sativa) embryo during grain germination. Endogenous sugar levels, accumulation of starch granules, and gene expression of a rice sucrose transporter (OsSUT1) were examined using rice embryos germinated with or without exogenous sugar supply. Starch granules remarkably accumulated in the cells around vascular bundles as a consequence of t...

An important development in the field of polysaccharide biochemistry has been the discovery of starch synthetase in beans, potatoes and corn seedlings by Leloir and coworkers•>. The enzyme was found to be closely bound to the starch granules and to catalyze the transfer of glucose moiety from UDP-glucose into polysaccharides containing linkage of the a-D-(H4) type. Subsequently, the granule-bou...