نتایج جستجو برای: ricin toxin b

تعداد نتایج: 943509  

Journal: :Cell 2010
Bahne Stechmann Siau-Kun Bai Emilie Gobbo Roman Lopez Goulven Merer Suzy Pinchard Laetitia Panigai Danièle Tenza Graça Raposo Bruno Beaumelle Didier Sauvaire Daniel Gillet Ludger Johannes Julien Barbier

Bacterial Shiga-like toxins are virulence factors that constitute a significant public health threat worldwide, and the plant toxin ricin is a potential bioterror weapon. To gain access to their cytosolic target, ribosomal RNA, these toxins follow the retrograde transport route from the plasma membrane to the endoplasmic reticulum, via endosomes and the Golgi apparatus. Here, we used high-throu...

Journal: :Journal of Immunology 2023

Abstract ARDS is a life-threatening respiratory condition associated with pulmonary vascular hyperpermeability and loss of aerated lung tissue. Ricin plant-toxin derived from the seeds Ricinus communis, irreversibly inactivates ribosomes arrests protein synthesis. Pulmonary exposure to ricin represents bona fide model for ARDS, characterized by inflammation massive neutrophil recruitment severe...

Journal: :Plant physiology 2001
L Frigerio N A Jolliffe A Di Cola D H Felipe N Paris J M Neuhaus J M Lord A Ceriotti L M Roberts

Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linker is removed. Here, we replicate these events in transfected tobacco (Nicotian...

Journal: :Cancer research 1993
S A Shah P M Halloran C A Ferris B A Levine L A Bourret V S Goldmacher W A Blättler

Anti-CD19 monoclonal antibody anti-B4 (IgG1) conjugated to the novel toxin-blocked ricin forms a potent immunotoxin, anti-B4-blocked ricin, that kills greater than 4.5 logs of CD19-positive cells in vitro after a 24-h exposure to a conjugate concentration of 5 x 10(-9) M (1.11 micrograms/ml). The efficacy of anti-B4-blocked ricin in vivo was assessed in survival models of SCID mice bearing eith...

Journal: :Bioscience, biotechnology, and biochemistry 2002
Ai Kageyama Izumi Kusano Tadashi Tamura Tatsuya Oda Tsuyoshi Muramatsu

We compared the abilities of ricin, diphtheria toxin, cycloheximide, and anisomycin to induce apoptosis, using human myeloid leukemia U937 cells at the concentration of each toxin at which almost complete protein synthesis inhibition was attained within 3 h. Among these toxins, anisomycin was found to be the most potent apoptosis inducer. After a 6-h exposure to anisomycin (1 microg/ml), nearly...

Journal: :Blood 1992
M L Grossbard A S Freedman J Ritz F Coral V S Goldmacher L Eliseo N Spector K Dear J M Lambert W A Blättler

Anti-B4-blocked Ricin (Anti-B4-bR) is an immunotoxin comprised of the anti-B4 monoclonal antibody (MoAb) and the protein toxin "blocked ricin." The anti-B4 MoAb is directed against the B-lineage-restricted CD19 antigen expressed on more than 95% of normal and neoplastic B cells. Blocked ricin is an altered ricin derivative that has its nonspecific binding eliminated by chemically blocking the g...

Journal: :Blood 1997
A C Goulet V S Goldmacher J M Lambert C Baron D C Roy E Kouassi

CD19 (B4) is a signal transduction molecule restricted to the B-cell lineage and the target of the immunotoxin anti-B4-blocked ricin (anti-B4-bR), which is composed of the monoclonal antibody (MoAb) anti-B4 and the modified plant toxin blocked ricin. To explore the influence of conjugation of blocked ricin to anti-B4 on functional activation of CD19, we investigated the effects of anti-B4-bR, a...

2012
Siu-Kei Chow Arturo Casadevall

Antibody therapy remains the only effective treatment for toxin-mediated diseases. The development of hybridoma technology has allowed the isolation of monoclonal antibodies (mAbs) with high specificity and defined properties, and numerous mAbs have been purified and characterized for their protective efficacy against different toxins. This review summarizes the mAb studies for 6 toxins--Shiga ...

2006
S. Ramakrishnan L. L. Houston

Pokeweed antiviral protein (PAP) and ricin A chain are potent inhibitors of protein synthesis that inactivate eukaryotic 60S ribosomal subunits. Immunotoxins were prepared by linking monoclonal anti-Thy 1.1 antibodies to PAP and ricin A chain through a disulfide bond. Both the conjugates were shown earlier to specifically inhibit protein synthesis of Thy 1.1-positive target leukemic cells (AKR ...

Journal: :Protein engineering, design & selection : PEDS 2004
Mark A Olson John H Carra Virginia Roxas-Duncan Robert W Wannemacher Leonard A Smith Charles B Millard

Previous attempts to produce a vaccine for ricin toxin have been hampered by safety concerns arising from residual toxicity and the undesirable aggregation or precipitation caused by exposure of hydrophobic surfaces on the ricin A-chain (RTA) in the absence of its natural B-chain partner. We undertook a structure-based solution to this problem by reversing evolutionary selection on the 'ribosom...

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