AIM To evaluate the biocompatibility of a resin-based endodontic filler (RealSeal) using the indirect cytotoxicity test. METHODOLOGY Human gingival fibroblasts were cultured ex vivo. Pellets of the materials to be tested were incubated for 24, 48, and 72 h at 37 degrees C under sterile conditions to obtain their eluates. The fibroblasts were exposed to either diluted (50%) or undiluted eluate...

INTRODUCTION A mandatory requirement of root canal therapy is that the obturation and restoration of the tooth must seal the root canals both apically and coronally to prevent leakage and percolation of oral fluids and to prevent recontamination of disinfected canals. Apicoectomy (apicectomy/root-end resection) with retrograde obturation is a widely applied procedure in endodontics, when all ef...

Mineral trioxide aggregate (MTA) has been shown to possess excellent sealing ability when tested with dye, bacteria, and a fluid filtration technique. Endotoxin, a component of the cell wall of Gram-negative bacteria, has been proposed to play a role in the pathogenesis of periradicular lesions. This study used a modified Limulus Amebocyte Lysate test for the presence of endotoxin as a tracer a...

bonding to dentin is a promising property, which can prevent leakage and improve the sealing ability of endodontic filling materials. resilon was developed and recommended initially because the existing root canal filling materials did not bond to root canal dentin. since its introduction in 2004, numerous reports have been published regarding various aspects of this material. this article aime...

A number of root-repair materials have been developed by various manufacturers, including Mineral Trioxide Aggregate (MTA) that has adequate biological and physical properties of a good root repair material [1,2]. MTA has been used for apexification, perforation repair, root resorption, and as a root-end filling material. The main ingredients of MTA are silicate oxide, tricalcium oxide, tricalc...

A review of the literature on the use of carrier based obturation materials focusing on Thermafil and Resilon based obturator (RealSeal 1) are presented in this article. The review addressed the history, apical leakage, coronal leakage, biocompatibility, sealing ability and clinical success of Thermafil and RealSeal 1. Based on the studies gathered, this review concluded that both treatment tec...

OBJECTIVES This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. MATERIAL AND METHODS Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. Th...