The circular dichroism (CD) spectrum of the Rev protein from HIV-1 indicates that Rev contains about 50% alpha helix and 25% beta sheet at 5 degrees C in potassium phosphate buffer, pH 3, and 300 mM KF. The spectrum is independent of protein concentration over a 20-fold range. At neutral pH, Rev is relatively insoluble but can be brought into solution by binding to its specific RNA binding site...

Human immunodeficiency virus type I encodes a regulatory protein, termed Rev, which is associated with the appearance of unspliced and partially spliced viral RNAs in the cytoplasm. Rev is believed to function via interaction with a sequence element in the env region of the viral RNA, termed the Rev-responsive element (RRE). In this study, we use a stably transfected, Rev-producing mouse cell l...

Epstein-Barr virus (EBV) expresses two transcription factors, Rta and Zta, during the immediate-early stage of the lytic cycle. The two proteins often collaborate to activate the transcription of EBV lytic genes synergistically. This study demonstrates that Rta and Zta form a complex via an intermediary protein, MCAF1, on Zta response element (ZRE) in vitro. The interaction among these three pr...

The aim of this paper is to form an analytical and critical framework consider the uses digital platforms in heritage field practices provide methodologies for user profiling based on identification local stakeholders their needs. Within context EU H2020 research project RURITAGE, a resource ecosystem (RRE) various integrated tools was created shaping addressing heritage-led knowledge bottom-up...

The aim of this paper is to promote quantum logic as one the basic tools for analyzing human reasoning. We compare it with classical (Boolean) and highlight role violation distributive law conjunction disjunction. It well known that nondistributivity equivalent incompatibility logical variables — impossibility assign jointly two-valued truth values these variables. A natural question arises whe...

Increased use of remotely sensed data is a key strategy adopted by the Forest Inventory and Analysis Program. However, multiple sensor technologies require complex sampling units and sampling designs. The Recursive Restriction Estimator (RRE) accommodates this complexity. It is a design-consistent Empirical Best Linear Unbiased Prediction for the state-vector, which contains all suffi cient sta...

In this paper, we consider a class of new accelerated multilevel aggregation methods using two polynomial-type vector extrapolation methods, namely the reduced rank extrapolation (RRE) and the generalization of quadratic extrapolation (GQE) methods. We show how to combine the multilevel aggregation methods with the RRE and GQE algorithms on the finest level in order to speed up the numerical co...

The HIV-1 Rev response element (RRE) is a ~350 nucleotide, highly structured, cis-acting RNA element essential for viral replication. It is located in the env coding region of the viral genome and is extremely well conserved across different HIV-1 isolates. It is present on all partially spliced and unspliced viral mRNA transcripts, and serves as an RNA framework onto which multiple molecules o...

Analyzing a logical structure of a sentence is important for understanding natural language. In this paper, we present a task of Recognition of Requisite Part and Effectuation Part in Law Sentences, or RRE task for short, which is studied in the research of legal engineering. The goal of this task is to recognize the structure of a law sentence. We investigate RRE task in both aspects: linguist...

We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dependence on the number and location of arginines in the different aminoglycosid...