Rhodnius prolixus, the principal Chagas disease vector, requires a blood meal to complete its moult cycle into the next stage. Allatotropins (ATs), a family of peptides first isolated from Manduca sexta, have been shown to regulate the biosynthesis of juvenile hormone, an insect growth and development hormone; however, ATs, being multimodal peptides, also exhibit myotropic effects on some insec...

INTRODUCTION Growth factors, cytokines, sex steroid hormones and glucocorticoids have differential and complex effects on skeletal metabolism. Recently, the presence of the glutamatergic (Glu) system in bone cells has provided new evidence for its possible role in bone physiology. Consequently, we have investigated the regulation of certain components of the Glu system by glucocorticoids in MG-...

Multiple myeloma (MM) is a currently incurable neoplasm of terminally differentiated B cells. The translocation and/or overexpression of c-MAF have been observed in human MM. Although c-MAF might function as an oncogene in human MM, there has been no report thus far describing the direct induction of MM by c-MAF overexpression in vivo. In this study, we have generated transgenic (TG) mice that ...

Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in China December 2019 and now has led to a global pandemic. The incidence is lower neonates. A five days old newborn infant got admitted with history of reluctance feeding less movement. On admission, the was found be lethargic, icteric, tachypnoeic, afebrile SPO2 90% throughout his h...

The Indian Council of Medical Research (ICMR) recently came up with an epidemiological definition for reinfection- a gap 102 days between two positive molecular or rapid antigen tests negative test in between. As it is not always feasible to do genomic analysis, this working can provide useful insights. Here we describe case series seven health care workers (HCWs) who turned the second time aft...

The glycine-N-acyltransferase (GLYAT) is well known to be involved in the detoxification of endogenous and exogenous xenobiotic acyl-CoA's in mammals. Unfortunately, the knowledge about the gene encoding GLYAT is very limited. Here we report a novel gene encoding a GLYAT member, designated as GLYATL1, which was 1546 base pairs in length and contained an open reading frame (ORF) encoding a polyp...

The full coding sequence for porcine erythropoietin receptor (EPOR) was elucidated using reverse transcription polymerase chain reaction (PCR) (rtPCR) and 39 and 59 rapid amplification of cDNA ends (RACE) procedures. Total RNA collected from Day 30 fetal liver was used as starting material. A 1843 bp sequence was obtained from which could be inferred a 509 amino acid protein which was 79–85% id...

Three methods were compared for the detection of prunus necrotic ringspot virus in herbaceous and woody plants: DAS-ELISA, nonisotopic dot-blot hybridization and reverse transcriptional polymerase chain reaction (RT-PCR). When purified virus preparations were used, the detection limit of the RT-PCR technique was 1·28 pg mL whereas nonisotopic molecular hybridization and DAS-ELISA allowed detect...

A DNA construct encoding cholera toxin B subunit (CTB) and an anti-diabetic peptide from human heat shock protein 60 (hsp60) as a fusion protein was produced and transferred into low-nicotine tobacco through Agrobacterium-mediated transformation. Integration of the fusion gene into the plant genome was confirmed by PCR. The transcriptional expression of the fusion gene was demonstrated by RTPCR...