The stability of trans-(Pt(NH3)2[d(CGAG)-N7-G,N7-G]) adducts, resulting from cross-links between two guanine residues at d(CGAG) sites within single-stranded oligonucleotides by trans-diamminedichloro-platinum(II), has been studied under various conditions of temperature, salt and pH. The trans-(Pt(NH3)2[d(C GAG)-N7-G,N7-G]) cross-links rearrange into trans-(Pt(NH3)2[d(CGAG)-N3-C,N7-G]) cross-l...

In this study, the effects of various concentrations of NaCl and KCl and partial replacement of NaCl withKCl on the growth characteristics of L. monocytogenes were evaluated. It was found that L. monocytogenescan grow in the presence of 1-9% NaCl and 1-11% KCl. The higher the concentration of salt used, the longerthe lag phase induced. In addition, it was observed that L. monocytogenes tolerate...

We demonstrate and analyze a novel desalination method which works by electrophoretically replacing sodium and chloride in feed salt water with a pair of ions, calcium and carbonate, that react and precipitate out. The resulting calcium carbonate precipitate is benign to health, and can be filtered or settled out, yielding low ionic strength product water. The ion exchange and precipitation emp...

Very small amounts of adrenocorticosteroids are synthesized by brain tissue in vitro. While there is evidence suggesting that the synthesis of aldosterone in the brain may have a role in the hypertension of the Dahl salt-sensitive rat, the de novo synthesis of aldosterone or corticosterone within the brain of a living animal has not been demonstrated. We have used sensitive ELISAs to measure al...

The effect of salting by different salt composition on fatty acid profile studied in hot smoked Black and Caspian Sea sprat, Clupeonella cultriventris. After initial prepare activities, samples were exposed to brining process within two salt concentrations including of 100 % NaCl (treatment 1 or T1) and 75 % NaCl- 25 % KCl (treatment 2 or T2) under 15% brine solution for 3 hrs. The smoking proc...

The effect of replacement of negatively charged amino acid residues on the function of Na+ transport proteins of the salt-tolerant yeast Zygosaccharomyces rouxii was examined by heterologous expression of mutant proteins in a strain of Saccharomyces cerevisiae, RH16.6, that lacks native Na+-ATPase activity due to null mutations of ENA1, ENA2, ENA3, and ENA4. Mutants of Na+/H+ antiporter gene (Z...

Decrease in experimental sensitivity of cryoprobe experiments for salty samples, attributed to increased sample conductivity, has been a long-standing issue in protein NMR. Salt concentration can not be simply reduced as this often leads to protein aggregation. A simple and inexpensive solution to this problem is demonstrated here. We show that even for proteins prone to aggregation, the tradit...

Halophilism has been of considerable interest since the report of Farlow (1880) on the reddening of salted fish. Any industry using either a salt packing process or a salt brine must be concerned with the control of halophiles. Considerable work has been done, therefore, with the isolation, identification, and control of these organisms. Stuart, Frey, and James (1933), Gibbons (1936), Lefevre a...

Salt reabsorption by the human sweat duct is markedly reduced in cystic fibrosis (CF). We used fluorescence-digital imaging microscopy in combination with a halide-specific fluorescent dye [6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ)] to determine if this defective salt reabsorption is referable to a reduced plasma membrane Cl- permeability of the epithelial cells that line the sweat duct. Swe...

(a) The responses of the vomeronasal organ to general odorants in the turtle, Geoclemys reevesii, were measured by recording the accessory olfactory bulbar responses. The threshold concentrations of the vomeronasal responses to various odorants were similar to those in main olfactory bulbar responses, indicating that vomeronasal cells lacking cilia and olfactory cells having many cilia have sim...