tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read the commentary by Dr. Ferlin with interest (1). The author first stated the drawbacks of semen analysis in the diagnosis of male infertility and the need for sperm function tests. It is followed by illustrating the limitation of the current evidence in supporting the routine use of sperm DNA fragmentation (SDF...

To evaluate whether the sperm DNA fragmentation affects male pronucleus epigenetic factors, semen analysis was performed and DNA fragmentation was assessed by the method of sperm chromatin structure assay (SCSA). Human-mouse interspecies fertilisation was used to create human male pronucleus. Male pronucleus DNA methylation and H4K12 acetylation were evaluated by immunostaining. Results showed ...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We are delighted to read the insightful commentary by Dr. Evenson (1), a well-known pioneer in the field of SDF testing, in response to the practice recommendations on clinical utility of sperm DNA fragmentation (SDF) testing by Agarwal et al. (2). The process of protamination during spermiogenesis was discovered as e...

The aim of this study was to analyse semen quality parameters fresh-extended porcine imported from Denmark through prolonged importing regulations and transport fertility performance in sows inseminated four days post collection. Semen analysed immediately upon the arrival (4 collection) using computer-assisted sperm analyses (CASA), cytomorphology flow cytometry, were later same day. mean conc...

This paper reports a case of recurrent miscarriage in a patient affected by a variant phenotype of sperm macrocephaly syndrome (SMS). SMS is usually related to specific sperm characteristics (large head, multiple tail) and homozygous mutations in the aurora kinase C gene (AURKC). However, the present case observed large-headed spermatozoa with no flagellar abnormalities and no mutations detecta...

Abstract Background The routine semen analysis fails to detect sperm DNA damage which contributes the majority of male factor infertility. Sperm fragmentation test (DFI) measures damage. Blastocyst formation is an important step in IVF ± ICSI. At present, literature lacks any data that correlates DFI and blastocyst formation. Main body abstract We searched MEDLINE other databases till 2020 for ...