Biochemical analysis of proteins relies on accurate quantitation of protein concentration. This unit describes how to perform commonly used protein assays, e.g., Lowry, Bradford, BCA, and UV spectroscopic protein assays. The primary focus of the unit is assay selection, emphasizing sample and buffer compatibility. Protein assay standard curves and data processing fundamentals are discussed in d...

The present study aimed to investigate the effect of N-Ethylmaleimide (NEM) on evaluation disulfide formation in oxi dized myofibrillar proteins during sample preparation non-reducing SDS-PAGE procedure. For this purpose, extracted were oxidized firstly via a Fenton oxidation reaction, and non-oxidized used as control. Before running SDS-PAGE, preparation, these prepared according three dif fer...

Koro kratok fermentation used Rhizopus oligosphorus was one of Indonesian’s society methods to consumed kratok. produced proteolytic enzyme which can be break down complex protein simple protein. Protein degradation observed based on the time koro Fermentation times were 0 hours, 12 24 36 48 72 96 hours and 120 fermentation. Parameters included activity, growth mycelium, dissolved protein, conc...

SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conducted using SDS-PAGE. From the quantitative analysis to the electrophoresis bands of BSA and its hydrolysis products in SDS-PAGE pattern, the change of trypsin activity was determined...

HIGHLIGHTS Ellagic acid (EA) reduces oxidative damage and thus it can protect S. cerevisiae against to ROS. SDS-PAGE results reveal that EA induces total protein synthesis. cell development MDA levels increases GSH levels.

background: xanthomonas citri subsp. citri (xcc), the causative agent of bacterial citrus canker, has affected citriculture worldwide. varieties of means have been used to minimize its devastating effects, but no attention has been given to bacteriocins.objectives: here and for the first time, we report the isolation and characterization of two novel bacteriocins.materials and methods: secretom...

The focus of the present study is to determine proteins responsible for oxidative and toxic stress response in proliferating stationary phase (G0) cultures. Therefore, yeast Saccharomyces cerevisiae was treated with drug compounds (H2O2, menadione, zeocin, ibuprofen) both phases. These substances were chosen redox status yeast. S. appeared employ different strategies ensure their antioxidant de...

SSPs (Seed Storage Proteins) of the 11S type have been preliminary characterized in the seeds of the argan tree, an endemic species from Morocco. Protein extracts from mature seeds were prepared by using different solutions in order to assess the solubility of the major protein forms. SSPs of the 11S type were classified as albumins according to the further SDS-PAGE analysis of these extracts. ...