We describe a von Willebrand disease (VWD) variant characterized by the persistence of von Willebrand factor (VWF) propeptide as a result of a C>T transition at nucleotide 2527 in exon 17 of the VWF gene. This mutation, which was present in the proband and his father, predicts the substitution of Cys for Arg at position 760 of pre–pro-VWF, 4 residues before the propeptide cleavage site belongin...

Urinary protein excretion occurs in neonates of many animal species, as well as in human neonates. However, the incidence, dynamics, and mechanism of proteinuria have not been unambiguously explained. The aims of this study were to investigate into excretion of selected protein fractions of molecular weight less than 69 kDa (LMW), evaluation of intensity and dynamics of changes during the first...

Cow milk, a highly nutritious food item, is amenable to quality degradation due to lipolysis and proteolysis, as a sequel to the growth of spoilage microorganisms, particularly under ambient condition in tropical countries, with consequential loss of aesthetic appeal and product output. Prescription antibiotics used for milk preservation have many undesirable side effects on the health of consu...

In order to distinguish the interactions between low oxygen and fruit developmental stages, preclimacteric and ripening-initiated with propylene avocado fruit were exposed to different low oxygen levels and the steady-state levels of protein and mRNA accumulation of selected hypoxic and ripening genes were investigated. In addition, the patterns of translatable mRNA were studied by 2D IEF/SDSPA...

Chronic renal disease (CRD) is a serious condition associated with mortality, and decreased quality of life. Moreover, patients of CRD are often suffering from hepatic insufficiency (HI). The plasma protein profile could be used as a predictor, as well as a diagnostic marker of preoperative liver and renal failure. The present study was carried out to evaluate the fluctuation in the plasma prot...

BACKGROUND we successfully reconstituted the gene from group 1 allergens of dust mites, and obtained a body of shuffled genes. In order to verify the prediction on the chimeric gene, we tentatively cloned R8 into the vector that was prokaryoticly expressed, purified and assessed for its bio-activities. METHODS the expressed product was detected by SDSPAGE and the target protein was purified. ...

background: phosphatidate phosphohydrolase (pap) catalyzes the dephosphorylation of phosphatidic acid to yield p i and diacylglycerol. two different forms of pap have been reported in rat hepatocyte: pap 1 that participates in the synthesis of phospholipids and triacylglycerols and pap 2 which is involved in lipid signaling pathways. two isoforms of pap 2 are pap 2a and pap 2b . this study exam...

Additional to industrial use of xylanase in kraft pulp production, thermostable xylanase has wider application in fishery, piggery, cattle food and human food. In this research, 1-4-β-Dendoxylanase was isolated from liquid state cultures of Bacillus brevis containing wheat straw as carbon source. Xylanase was purified to apparent homogeneity by gel filtration and ion exchange chromatography. Th...