We have established cell lines from six human squamous cell carcinomas (SCC) of the epidermis and tongue, using culture methods previously developed for clonal growth and serial cultivation of normal keratinocytes. The SCC lines all form rapidly growing, well-differentiated SCC's or progressively growing squamous cysts in nude mice. In contrast to normal keratinocytes, SCC cells form unstratifi...

Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues. In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus roseus, isolated from soil samples of Gangotri glaciers, Western Himalayas. Among various substrate tested, groundnut oil cake (GOC) favored maximal yield of lip...

INTRODUCTION Our various previous findings have shown the suitability of norfloxacin in the treatment of bacterial infections and burn wounds in alone as well as in combination with Curcuma longa in various topical (ointments, gels, and creams) and transdermal drug delivery systems. AIMS AND METHODS Keeping these facts in consideration, we have made an another attempt to prepare semisolid for...

The aim of the study was to select the optimal composition of semisolid using natural materials, and to evaluate the quality of such preparation. Investigation of rheological properties, evaluation of sensory characteristics, detection and identification of phenolic acids using high-performance liquid chromatography (HPLC) were performed. The results of the rheological investigations and the st...

The evaluation of drug permeation/penetration of semisolid formulations into animal skin can be useful to supplement the pharmaceutical equivalence. This paper describes the in vitro assessment of acyclovir (ACV) into porcine skin from commercial formulations with etermination of drug concentration in different layers of cutaneous tissue to correlate with effective antiviral concentration in or...

The interconversion of atmospheric organic particles among solid, semisolid, and liquid phases is of keen current scientific interest, especially for particles of secondary organic material (SOM). Herein, the influence of phase on ammonia uptake and subsequent particle-phase reactions was investigated for aerosol particles of adipic acid and α-pinene ozonolysis SOM. The nitrogen content of the ...

A semisolid tetrazolium salt agar medium developed for testing sensitivity to KCN is easier to interpret, has a longer storage life, and is less critical in the size of inoculum than is Moeller KCN broth medium.

One of the most significant issues in pharmaceutical industries, prior to commercialization of a pharmaceutical preparation is the "preformulation" stage. However, far too attention has been paid to verification of the software assisted statistical designs in preformulation studies. The main aim of this study was to report a step by step preformulation approach for a semisolid preparation based...

The transformation of primary rat cells into established cell lines by eimian virus 40 has been monitared using the different restrictive assays of colony formation in sparse culture, dense colony or focus formation on a confluent cell sheet, and colony formation in semisolid medium. Primary embryonic rat cell cultures are considerably less susceptible to infection and subsequent transformation...