OBJECTIVE The purpose of this study was to investigate the efficacy of (Pro Seal) sealant in preventing enamel decalcification in-vivo and compare its effect with fluoride varnish and unfilled sealant using atomic force microscopy. MATERIALS AND METHODS Eight orthodontic patients who were candidates for extraction of all first premolars for orthodontic treatment were recruited to this study. ...

Bone decalcification is a time-consuming process. It takes weeks and preservation of the tissue structure depends on the quality and velocity of the demineralization process. In the present study, a decalcification methodology was adapted using microwaving to accelerate the decalcification of rat bone for electron microscopic analysis. The ultrastructure of the bone decalcified by microwave ene...

The effects of a combined fixing and decalcifying solution, trichloroacetic acid, on prepared bone marrow specimens were evaluated to try to improve the speed of trephine biopsy processing in our laboratory. The morphological preservation obtained immediately after decalcification was complete was assessed, and also after prolonged immersion in each of two solutions. Trichloracetic acid was mad...

To facilitate the molecular analysis of tissues in adult zebrafish, we tested eight different fixation and decalcification conditions for the ability to yield DNA suitable for PCR and tissue immunoreactivity, following paraffin embedding and sectioning. Although all conditions resulted in good tissue histology and immunocytochemistry, only two conditions left the DNA intact as seen by PCR. The ...

The objective of this study was to investigate the effect of elastomeric and stainless steel ligatures on the microbiology of local dental plaque. Clinical reports have shown that patients who receive orthodontic treatment are more susceptible to enamel white spot formation. Metallic orthodontic brackets have also been found to inflict ecologic changes in the oral environment, such as decreased...

A major degradation scenario within safety assessment analysis of nuclear waste repositories is decalcification of cementitious materials due to long-term contact with groundwater. Understanding the changes in Physical and Chemical properties of cementitious barriers is of high importance. In order to decrease the uncertainty in the assessments due to extrapolating short term data sets, acceler...

The aim of the study was to investigate the prevalence of enamel de-calcification following fixed orthodontic treatment and to discuss the various current treatment options available to avoid it. Group A (experimental) comprised a total of 47 (Male: Female Ratio 23:24) post-orthodontic treatment patients, compared to Group B (control) with a total of 49 (Male: Female Ratio 29:20) post-orthodont...

This prospective, in vivo study compared bond failure and enamel decalcification with a cyanoacrylate bracket bonding system (SmartBond, Gestenco International, Gothenburg, Sweden) and a traditional light-cured composite system (Light Bond, Reliance Orthodontic Products, Itasca, Ill). A total of 327 teeth were evaluated after a period of 12 to 14 months; 163 experimental teeth were bonded with ...

Mitral valvular heart disease increases sharply with age as a consequence of the predominance of degenerative valvular diseases, as well as being secondary to an increase in congestive heart failure. Nevertheless, owing to the rise in patients’ life expectancy, redo valve surgery is increasingly necessary and owing to technical aspects [1] or poor clinical conditions [2], it is associated with ...

SUMMARY Treatment with fixed orthodontic appliances can cause enamel demineralization by increased biofilm adhesion. The purpose of the present study was to investigate whether a polytetrafluoroethylene (PTFE) coating reduces biofilm formation on orthodontic brackets. One PTFE-coated bracket and one uncoated stainless steel bracket were bonded symmetrically on the first or second (four maxillar...