This work presents an inertial microfluidic band-pass filter, enabling continuous separation of three sizes of particles or cells with <3μm difference in diameter. The design uses inertial forces to focus particles into two bands along side walls. Two chambers with side outlets are cascaded in series to generate microvortices for continuous band-pass separations. Particles with diameters 23μm, ...

Microfluidic particle separation arrays are capable of passive sorting of microparticles or cells by size while avoiding blockage. Despite the usefulness of boundaries for concentration and parallel integration of arrays, separation efficiency is severely degraded in the areas adjacent to the boundaries due to the aberrant fluid flow found there. This letter shows how to eliminate this problem ...

We present an integrated thermoplastic elastomer (TPE) based multilayer microfluidic device with an embedded peristaltic micropump and through-holes membrane for high throughput particle sorting and separation. Fluidic and pneumatic layers of the device were fabricated using hot-embossing lithography and commercially available polycarbonate membranes were succcessfully sandwiched between two th...

Simultaneous recording from large numbers of neurons is a prerequisite for understanding their cooperative behavior. Various recording techniques and spike separation methods are being used toward this goal. However, the error rates involved in spike separation have not yet been quantified. We studied the separation reliability of "tetrode" (4-wire electrode)-recorded spikes by monitoring simul...

Isolation of different cell types from one sample by fluorescence activated cell sorting is standard but expensive and time consuming. Magnetic separation is more cost effective and faster by but requires substantial effort. An innovative pluriBead-cascade cell isolation system (pluriSelect GmbH, Leipzig, Germany) simultaneously separates two or more different cell types. It is based on antibod...

This paper describes a simple microfluidic sorting system that can perform size profiling and continuous mass-dependent separation of particles through combined use of gravity (1 g) and hydrodynamic flows capable of rapidly amplifying sedimentation-based separation between particles. Operation of the device relies on two microfluidic transport processes: (i) initial hydrodynamic focusing of par...