タンパク質のPEG化は安定性を向上することが知られているが，反応制御は難しく，複数の修飾異性体が生成する．これらの修飾体の分離には，イオン交換クロマトグラフィー（IEC）が用いられる．タンパク質に対して構築されたIECの分離機構モデルをPEG化タンパク質に適用することができれば，分離条件を合理的に決定可能となる．このためmono-PEG修飾体としてPEG化BSAを，random-PEG修飾体としてPEG化lysozymeを用い，それらの溶出時の塩濃度勾配と溶出塩濃度を，機構モデルを用いて解析した．いずれのPEG修飾体においてもモデルの適用が可能であり，担体上の結合部位の数は未修飾のものとほぼ同じ値をとることがわかった．また，機構モデルから算出される分配係数に基づき，移動相塩濃度を設定すると，PEG化と同時にカラムから反応生成物を脱離させることや，リサイクル型の連続反応分離操作も可能で...

Nitrogen is produced mainly from its most abundant source, the air, using three processes: membrane, pressure swing adsorption (PSA) and cryogenic. The most common method for evaluating a process is using the selection diagrams based on feasibility studies. Since the selection diagrams are presented by different companies, they are biased, and provide unsimilar and even controversial results. I...

in this study, response surface methodology was used to determine the optimum raw sugar beet juice purification process conditions using bentonite to produce inverted liquid sugar. for this purpose, impact of factors on the purification process such as bentonite concentration (1-5 gr/li), ph (3.5-6) and temperature (35-95 ˚c) was investigated. for each response, a second-order polynomial model ...

toluidine blue o (tbo) is a cationic dye which is extensively used in the industries. in the present paper a simple and efficient wet chemical method was introduced for removal of tbo from waste aqueous solution. magnetic multi-walled carbon nanotubes were synthesized using commercially available multi-wall carbon nanotubes and magnetic iron oxide nanoparticles which were examined for removal o...

in order to investigate the gas separation ability of a column packed with nanozeolitic material, nano nay zeolite was synthesized and granulated. these uniform granules packed in a chromatographic column were utilized for separation of xe and kr under various operating conditions. with regards to the response peaks obtained from trace injections of xe and kr into the column, the first and seco...

direct contact membrane distillation (dcmd) which emerges as an alternative separation technology can effectively perform a colloidal separation process under thermal driven force. dcmd is capable of extracting pure water from aqueous solutions containing non-volatile nanoparticles through the hydrophobic microporous membrane when a vapour pressure difference was established across the membrane...