In 1943 Bruger (1) proposed a fractional cephalin-cholesterol flocculation test to be used as an index of increasing or decreasing hepatic dysfunction. This modified test consisted in the use of increasing dilutions of serum with saline, noting the flocculation according to the procedure originally described by Hanger. The data presented in support of the modified test do show that, when it is ...

Aquatics are a source of bioactive compounds that these compounds have different properties such as antimicrobial activity. In this study, antibacterial activity of methanol, chloroform and hexane extracts from body wall, gonad and intestine of sea cucumber Holothuria leucospilota was evaluated against Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Bioactive compounds of b...

Stroke volume was determined from the pulmonary artery pressure record by application of the pulse contour method. Pulmonary artery pressure records were obtained in 17 patients using a high fidelity catheter-tip pressure transducer and simultaneous measurements of cardiac output were obtained from indicator dilution curves. The formula used was SV = KP sa (i plus T s/Td) where Psa is the plani...

AIMS A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was added to each well. For resuscitation of heat-injured (55 degrees C for 10 min)...

objective: this study designed to test the antibacterial potency of enrofloxacin (enr) and essential oils from zataria multiflora boiss (zeo) and trachyspermum copticum (teo)on aeromonas hydrophila. material and methods: the antibacterial potency of test compounds was determined by several methods including the inhibition zone diameter determination, microbroth dilution method and colorimetric ...

When antibiotic sensitivity of microorganisms is tested by agar dilution, single antibiotics are incorporated in solid media and 'spot' inocula of test strains are applied to the antibiotic media and to a plate without antibiotic. The usual method of quality control is to include control strains of known sensitivity.1 However, control strains are usually sensitive to a number of antibiotics, an...

This paper demonstrates a facile method of generating precise serial dilutions in the form of droplets on an open surface platform. The method relies on the use of surface energy traps (SETs), etched areas of high surface energy on a Teflon coated glass substrate, to assist in the magnetic manipulation of droplets to meter and dispense liquid of defined volumes for the preparation of serial dil...

aquatics are a source of bioactive compounds that these compounds have different properties such as antimicrobial activity. in this study, antibacterial activity of methanol, chloroform and hexane extracts from body wall, gonad and intestine of sea cucumber holothuria leucospilota was evaluated against bacillus subtilis, pseudomonas aeruginosa and staphylococcus aureus. bioactive compounds of b...

The soft-agar overlay technique was originally developed over 70 years ago and has been widely used in several areas of microbiological research, including work with bacteriophages and bacteriocins, proteinaceous antibacterial agents. This approach is relatively inexpensive, with minimal resource requirements. This technique consists of spotting supernatant from a donor strain (potentially harb...