The small size of microand nanomechanical resonators, combined with the slow decay of their vibrational modes, enable numerous applications of these devices—from the studies of macroscopic quantum phenomena [1, 2], to mass detection with single-atom sensitivity [3, 4], to imaging with single-atom and single-spin resolution [5]. An important feature of nanoresonators is the vibration nonlinearit...

A frequent question among clinical biochemists is how sensitive analytical techniques should be in the context of clinical diagnostics. Currently, the analyte concentrations usually encountered in clinical chemistry range from 10 to 10 mol/L. By far, the most sensitive nonamplification techniques used in the clinical laboratory are based on noncompetitive immunological assays. Is there any need...

EEG contains a wealth of physiological, psychological and pathological information, EEG signal analysis and processing are either in clinical of some brain disease diagnosis and treatment, or in the cognitive science research fields are very important. According to the basic characteristics of EEG signal, discusses the EEG signal acquisition principle, method and the design train of thought. Pr...

RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detec...

BACKGROUND High sensitivity of analysis is constantly in demand in biomedical research and clinical diagnosis. In recent years aptamer-based analytical methods have been developed for protein detection. We developed a cascade signal amplification strategy based on molecular switches and aptamers to improve protein detection. METHODS Our cascade signal amplification strategy based on molecular...

Electromyographic (EMG) signals have been widely employed as a control signal in rehabilitation and a means of diagnosis in health care. Signal amplification and filtering is the first step in surface EMG signal processing and application systems. The characteristics of the amplifiers and filters determine the quality of EMG signals. Up until now, searching for better amplification and filterin...

The bottom-up design of protein-based signaling networks is a key goal of synthetic biology; yet, it remains elusive due to our inability to tailor-make signal transducers and receptors that can be readily compiled into defined signaling networks. Here, we report a generic approach for the construction of protein-based molecular switches based on artficially autoinhibited proteases. Using struc...

G protein activation by membrane-bound receptors initiates a chain reaction that amplifies cellular responses to external signals. In retinal photoreceptors, amplification by the phototransduction cascade is regulated by quickly switching off the visual pigment that acts as the receptor for light.

F. Schmid, M. Tsotsalas, M. Kuhlmann, C. Höltke, C. Bremer, W. Heindel, A. Guerrero-Martinez, J. Keupp, M. Schäfers, L. De Cola, and C. Faber Institute for Clinical Radiology, University Hospital Münster, Münster, Germany, Physikalisches Institut and NRW Graduate School of Chemistry, Westfälische Wilhelms-Universität Münster, Münster, Germany, European Institute for Molecular Imaging, Westfälis...