Male accessory gland secretory proteins in seven members of Drosophila nasuta subgroup were analyzed by SDS-PAGE in combination with different staining techniques such as CBB-R250, Silver, PAS, PAS-silver and zinc-imidazole reverse staining. Based on coomassie blue patterns the protein fractions could be classified in to 3 major groups namely group I, group II as well as group III; with high mo...

CS preparations for topical and/or oral use were subsequently developed to overcome the astringency of, and staining by, simple silver salts[2] while retaining the long-known antiseptic properties of metallic silver.[3] The older preparations, pre-1910, usually contained high levels of oxidized silver (Ag+), not precipitated by isotonic salt solutions, made from silver nitrate and various carri...

Human monocytes show a high affinity for vascular endothelium both in vitro and in vivo. To explore monocyte-endothelial interaction in greater detail, we have developed a new in vitro model for growth of human endothelial cells (EC). Human umbilical vein EC (HUVEC) cultured upon collagen gels form confluent monolayers of EC that bind silver at their intercellular border similar to cells in sit...

Proteins absorbed directly onto nitrocellulose membranes were stained with amido black, ponceau S, colloidal silver, or Coomassie blue and solubilized in dimethyl sulfoxide and the absorbance was measured spectrophotometrically. The optimal wavelength of each dye/protein/nitrocellulose solution was found to be at 625, 529, 420, and 600 nm, respectively. A linear relationship was found between t...

Metaphase chromatids are believed to consist of loops of chromatin anchored to a central scaffold, of which a major component is the decatenatory enzyme DNA topoisomerase II. Silver impregnation selectively stains an axial element of metaphase and anaphase chromatids; but we find that in earlier stages of mitosis, silver staining reveals an initially single, folded midline structure, which sepa...

AIMS To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. METHODS Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxid...

Objective: To synthesize silver nanoparticles extracellularly using Bacillus sp. isolated from soil sample. Methods: Bacteria capable of synthesizing silver nanoparticles extracellularly were screened by treating culture supernatant with silver nitrate solution. The potential isolate was isolated on nutrient agar medium and identified by Gram staining and biochemical tests. Results: One of the ...

The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their d...