An ultrasensitive electrical detection method of nucleic acids has been developed on a nanogapped biosensor. In this study, peptide nucleic acid (PNA) probes were immobilized in the gaps of a pair of finger microelectrodes first and were then hybridized with their complementary target DNA. After that, pectin molecules were introduced into the DNA strand via zirconium-phosphate and zirconium-car...

An ad hoc network is a rapidly deployable wireless network that has no centralized control mechanism. Mobile devices in an ad hoc network request a variety of data types, including text and multimedia data. Different types of data need to be treated with different qualities of service. The implementation of service differentiation in wireless networks is very difficult because of device mobilit...

This paper reports the synthesis of silver nanoparticles coated with porous silica (Ag@Silica NPs) using an assisted laser ablation method. This method is a chemical synthesis where one of the reagents (the reducer agent) is introduced in nanometer form by laser ablation of a solid target submerged in an aqueous solution. In a first step, a silicon wafer immersed in water solution was laser abl...

We describe a method based on fluorescence in situ hybridisation (FISH) that allows the identification of individual cells by electron microscopy. We hybridised universal and specific fluorescein-labelled oligonucleotide probes to the ribosomal RNA of prokaryotic microorganisms in heterogeneous cell mixtures. We then used antibodies against fluorescein coupled to sub-nanometer gold particles to...

The biological activity of the silver nanoparticles was tested in the primary cultured human periodontal ligament cells. Two types of silver nanoparticles (colloidal silver and plasma silver) were included in this study and MTT assay, cytotoxicity assay, and apoptosis assay were done. In results, the colloidal silver showed higher inhibition on the cellular proliferation than the plasma silver ...

Gold and silver nanoparticles (NPs) were prepared in water, acetonitrile and isopropanol by laser ablation methodologies. The average characteristic (longer) size of the NPs obtained ranged from 3 to 70 nm. 4-Aminobenzebethiol (4-ABT) was chosen as the surface enhanced Raman scattering (SERS) probe molecule to determine the optimum irradiation time and the pH of aqueous synthesis of the laser a...

Silver has a long and intriguing history as an antibiotic in human health care. It has been developed for use in water purification, wound care, bone prostheses, reconstructive orthopaedic surgery, cardiac devices, catheters and surgical appliances. Advancing biotechnology has enabled incorporation of ionizable silver into fabrics for clinical use to reduce the risk of nosocomial infections and...

In the past year we have completed measurements of polarized neutron–polarized He scattering in the few MeV region. Measurements of the total cross-section differences ∆σT and ∆σL have been obtained for neutrons with incident energies 1-10 MeV. The results are sensitive to the excited state structure of the alpha particle and may be compared to phase-shift predictions based on a number of analy...