In light of the proliferation of channels and receptors that are being cloned from the mammalian CNS it is a major challenge to understand their different roles in signalling and synaptic transmission within the brain. The patch-clamp technique developed by Neher, Sakmann and co-workers 1-3 has allowed successful studies of ionic currents and the underlying single-channel activity in the small ...

Corpus striatum neurons acutely dissociated from the brains of young adult rats had membrane surfaces suitable for G omega-seal recording. Whole-cell current-clamp and voltage-clamp recordings indicated that the cells remained electrically excitable after dissociation. Cell-attached recordings frequently revealed single-channel openings in the presence of dopamine or of the D2 dopamine agonist ...

This paper presents the recognition of fetal ECG signals from a single-channel recording. The Continuous Wavelet Transform generates two-dimensional representation single-dimension input containing electrocardiogram waves, which are accurately located and isolated. By segmenting this 2D using Otsu's method, continuous wavelet transform each wave may be estimated. temporal expression is then reb...

Seizure duration was estimated using a single channel EEG recording during ECT. Seizure endpoint was defined as the point of last spike. Three psychiatrists independently rated forty seven EEG records. Seizure endpoint could be reliably identified. The reliability coefficient between three raters was 0. 92, indicating high agreement among raters. Modal difference between any pair of raters as w...

Multiple distinct K+-selective channels may contribute to action potential repolarization and afterpotential generation in chick ciliary neurons. The channel types are difficult to distinguish by traditional voltage-clamp methods, primarily because of coactivation during depolarization. I have used the extracellular patch-clamp technique to resolve single-channel K+ currents in cultured chick c...

This paper develops coding and signal processing approaches for a novel optical recording channel that arises from electron-trapping phosphor materials. The recording medium allows multiple reads and writes, and one important feature is that the read process serves to erase the disk. This feature would enable vendors of prerecorded video to provide customers with one-time services. For applicat...

The inferior colliculus, the primary nucleus in the mammalian auditory midbrain, occupies a central position in the ascending auditory pathway. Nearly all ascending neural pathways converge and synapse in the central nucleus of the inferior colliculus (ICC). Further, the anatomical arrangement of axons and neurons in the ICC suggests the existence of functional regions which may play a role in ...

Present whole-cell patch-clamp methodology has only moderate consistency and throughput, rendering impractical functional measurements on large numbers of ion channel ligands or on large numbers of unknown or mutant channel genes. In the population patch clamp (PPC) described herein, a single voltage-clamp amplifier sums the whole-cell currents from multiple cells at once, each sealed to a sepa...

The transient receptor potential vallinoid type 4 (TRPV4) is a calcium entry channel known to modulate vascular function by mediating endothelium-dependent vasodilation. The present study investigated if isolated cerebral arterial myocytes of the Fawn Hooded hypertensive (FHH) rat, known to display exaggerated KCa channel current activity and impaired myogenic tone, express TRPV4 channels at th...