mastitis is one of the most serious and costly diseases affecting dairy cattle production. in the present study, effects of a lactoferrin gene polymorphism (intron 6) on milk somatic cell count (scc) and subclinical mastitis was investigated in 121 holstein dairy cattle. two alleles of a and b and two genotypes of aa and ab were found in an ecori recognized single nucleotide polymorphism in int...

The aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismPCR-RFLP genotyping method for the AY_376689:c.773C>T single nucleotide polymorphism SNP of the equine PRKAG3 gene, related to muscle performance, as well as to characterize this SNP and a second polymorphism, AAWR_02017454:g.121684T>C of the SPATA1 gene, related to male fertility, i...

The present study was carried out to investigate the association of C/T single nucleotide polymorphism(SNP)in exon 5 of stearoyl-CoA desaturase 1 (SCD1) gene and A/C SNP in the 3' untranslated region of oxidized low density lipoprotein receptor 1 (OLR1)gene with milk production traits in Iranian Holstein dairy Cattle. The blood samples of 153 (for OLR1) and 308 (for SCD1) dairy cattle from thre...

Gene polymorphism refers to a variation in DNA sequence that occurs population with frequency of 1% or higher. Polymorphism may be single nucleotide (SNP) some repetitive sequences (length polymorphism). Several methods can used analyze polymorphism, included Polymerase Chain Reaction (PCR), sequencing from the conventional method more sophisticated such as Next Generation Sequencing (NGS), flu...

Identification of polymorphism is the key for genetic mapping, diagnosis and marker-assisted selection. Restriction fragment-length polymorphisms (RFLPs) and short tandem repeats (STRs), also known as microsatellites, are commonly used genetic markers. Until discovery of STRs, RFLPs were used for genetic mapping, but bi-allelic polymorphism of RFLP limited its use as a marker. Although STRs are...

The diacylglycerol acyltransferase 1 gene (DGAT1) was identified as a strong candidate gene affecting mutton quality traits in sheep. Single nucleotide polymorphism creates a single base mutation (C to T) in AGCT site of endonuclease AluI. DGAT1 is one of the candidate genes to improve carcass characteristics in feedlot animals. In order to study area T487C in exon 17 of the DGAT1 polymorphism,...

Background: DNA markers are inevitable tools of human identification in forensic science. Single Nucleotide Polymorphisms (SNPs) are one category of these markers which is concerned to use especially in the case of degraded DNA because of their short amplicons. Objectives: Detection of highly informative SNPs by the criteria is the essential step to devel...

Genotype imputation from low-density to high-density (SNP) chips is an important step before applying genomic selection, because denser chips can provide more reliable genomic predictions. In the current research, the accuracy of genotype imputation from low and moderate-density panels (5K and 50K) to high-density panels in the purebred and crossbred populations was assessed. The simulated popu...

Background: Tssk2, a member of the testis specific serine/threonine kinase (TSSK) family, is expressed predominantly in the testis and crucial for the formation and function of the sperm cells in mouse. Targeted deletion of Tssk1 and 2 in male chimeric mice caused infertility due to haploinsufficiency of the genes. Therefore it is reasonable to postulate that mutations in its human homologue TS...