this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...

purification of extra-cellular cellulolytic enzymes from c e l l u l o mssp. strain 0, isolated from forest soil in the north of iran , was studied by using gel filtration and ion-exchange chromatography. two endo-glucanases (endo-i & endo-ii) and one exo-glucanase (exo-i) were purified to hornogenity . the purified enzymes had molecular weights of about 39.000,70.000 and 90.000 daltons for end...

The novelty of the present research consisted in study features heavy metals accumulation phytomass agricultural plants under conditions complex contamination podzolized chernozem (ashy soil) Ryazan region (Russia). Results vegetation experiments conducted on four crops—oats, black beans, buckwheat, and soybeans—were analyzed, which made it possible to assess ability these accumulate their depe...

Soil contamination by different hydrocarbons has rapidly expanded worldwide, surpassing the self-purification capacity of soils and increasing number contaminated sites. Although much effort been devoted to study effects diesel on geotechnical properties soil, there is still limited available information about it. Moreover, no maximum retention that soil can have its effect behavior soil. Thus,...

BACKGROUND AND OBJECTIVES Proline dehydrogenase (ProDH; 1.5.99.8) plays an important role in specific determination of plasma proline level in biosensor and diagnostic kits. The goal of this research was to isolate and characterize ProDH enzyme from Iranian soil microorganisms. MATERIALS AND METHODS Screening of L-proline degradative enzymes from soil samples was carried out employing enrichm...

We report on the development and validation of a simple microarray method for the direct detection of intact 16S rRNA from unpurified soil extracts. Total RNAs from Geobacter chapellei and Desulfovibrio desulfuricans were hybridized to an oligonucleotide array consisting of universal and species-specific 16S rRNA probes. PCR-amplified products from Geobacter and Desulfovibrio were easily and sp...

We have quantified ribose, rhamnose, arabinose, xylose, fucose, mannose, glucose, and galactose in soil by gas chromatography (GC) simultaneously after converting to aldononitrile acetate derivatives. A recommended single-hydrolytic step by 4M trifluoroacetic acid (TFA) at 105 1C for 4 h was more effective for releasing soil neutral sugars from non-cellulosic carbohydrates and better suited to ...

Efficient, nonselective methods to obtain DNA from the environment are needed for rapid and thorough analysis of introduced microorganisms in environmental samples and for analysis of microbial community diversity in soil. A small-scale procedure to rapidly extract and purify DNA from soils was developed for in-the-field use. Amounts of DNA released from bacterial vegetative cells, bacterial en...