The effect of rabbit antiidiotypic antibody raised against human IgG F(ab')2 anti-tetanus toxoid (TT) antibodies on the in vitro synthesis of TT-specific IgE antibody by peripheral blood lymphocytes (PBL) was examined in three subjects. Two of these subjects were allergic twins whose sera persistently contained IgE anti-TT antibodies. The third subject was a nonallergic individual who had a sli...

Allergic diseases affect approximately one-quarter to one-half of the average population under 50 years age in Central Europe. Due high proportion affected individuals, allergy testing needs be performed on a large scale, with sensitivity and specificity at low cost. Skin tests are most important diagnostic measure fulfilling these requirements: they can immediately and, quite contrast laborato...

We determined pollen specific IgE in tears and compared these results to the concentration of specific IgE in serum samples. We obtained tears (using Schirmer strips) and serum samples from subjects with Japanese cedar (Cryptomeria japonica) pollinosis, and tested for C. japonica pollen specific IgE using a quantitative ELISA. Time kinetic analyses through the pollen season showed that specific...

Measurement of allergen-specific immunoglobulin E (IgE) is a common practice in the investigation of allergy. It has not been possible to measure porcine IgE due to unavailability of anti-porcine IgE. This study was undertaken to purify and characterize porcine IgE from sera of allergic pigs, identify heterologous anti-IgE reactive with pig IgE and to use purified heavy (H) chain of porcine IgE...

BACKGROUND Immunoglobulin (Ig) E-mediated sensitization to N-glycans (cross-reactive carbohydrate determinants, CCDs) may induce double IgE reactivity to honeybee venom (HBV) and yellow jacket venom (YJV) in patients who are monosensitized to either of these venoms. Alcohol consumption is associated with increased IgE levels and possibly with sensitization to CCDs in the general population. O...

conclusions the characteristic features of ige-mediated food allergy are abrupt onset of clinical symptoms, which may result in a life-threatening events, and positive results of the majority of the paraclinical tests that mainly trace the specific ige to foods. moreover, non–ige-mediated food allergies present as chronic diseases and due to lack of proper diagnostic tests the similarity of the...

Prior findings linking allergy and cancer have been inconsistent, which may be driven by diverse assessment methods. We used serum specific immunoglobulin E (IgE) against common inhalant allergens that was assessed prior to cancer diagnosis in studying this association. We selected 8,727 Swedish men and women who had measurements of serum allergen-specific IgE and total IgE between 1992 and 199...

CONTEXT The diagnostic algorithm for human allergic disease involves confirmation of sensitization by detection of allergen-specific immunoglobulin E (IgE) antibody in individuals suspected of having allergic disease because of a history of allergic symptoms after known allergen exposure. Previous studies showed wide disparity among clinically reported allergen-specific IgE levels from differen...

Lithospermum erythrorhizon (L. erythrorhizon) root is used in traditional medicine for its anti-inflammatory, antibacterial, and antioxidant properties. However, no studies have examined impact on allergic rhinitis (AR). Here, we explored the protective effects of L. immunoglobulin E (IgE)-stimulated RBL-2H3 cells an ovalbumin (OVA)-induced AR mouse model. In latter, nasal mucosal inflammation,...

aspergillus species (a flavus and a niger) are important sources of inhalant allergens. current  diagnostic  modalities  employ  crude  aspergillus  extracts  which  only  indicate  the source to which the patient has been sensitized, without identifying the number and type of allergens in crude extracts. we report a study on the identification of major and minor allergens of the two common air...