The separation of N-acetyl-beta-D-hexosaminidase isoenzymes from human tissues is used in the diagnosis and differential diagnosis of GM(2) gangliosidosis, since in type 1 the A isoenzyme is deficient and in type 2 both the A and B isoenzymes are deficient. Peripheral blood leucocytes are commonly used for these investigations, and the present study demonstrates that, in addition to these two i...

BY CELLULOSE ACETATE OR ACRYLAMIDE GEL ELECTROPHORESIS IT IS POSSIBLE TO SEPARATE THESE ALKALINE PHOSPHATASE ISOENZYMES FROM SERUM: [anode] fast liver, slow liver, placenta/Regan, bone, intestine, bile [cathode]. Heat or chemical inhibition can confirm the differentiation. Normal adult serum always contains slow-liver isoenzyme, and sometimes bone isoenzyme: the latter is always present in seru...

Two different isoenzymes of fructose-P2 aldolase can be resolved by chromatography of crude spinach leaf extracts on DEAE-cellulose columns. The acidic isoenzyme comprises about 85% of the total leaf aldolase activity. The two forms differ in primary structure as judged by their distinctive amino acid compositions, tryptic peptide patterns, and immunological properties. Only the acidic isoenzym...

Pleurotus ostreatus is a white rot basidiomycete that produces several extracellular laccase isoenzymes, including phenol oxidase A1b (POXA1b), POXA2, and POXC. POXC was the most abundant isoenzyme produced under all of the growth conditions examined in this study. Copper was the most efficient inducer of laccase activity among the putative inducers tested. The amounts of all of the previously ...

Diagnosis of injury to the myocardium is facilitated by information on the activities of creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) and lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 in serum, these isoenzymes being present in higher activities in the myocardium than in other tissues or in normal serum. The temporal relationships of these isoenzymes, total creatine kinase, total lactate...

Criteria for the retrospective diagnosis of acute myocardial infarction rely heavily on increases in lactate dehydrogenase (LD) isoenzymes. However, increases of LD isoenzyme activities are not specific for myocardial injury. Recently, increased concentrations of cardiac troponin I (cTnI) have been shown to be highly specific for myocardial damage and to have sensitivity comparable with that of...

the effects of different storage temperatures and times on germination rate, malondialdehyde (mda), superoxide dismutase (sod), peroxidase (pod) and catalase (cat) activities in jatropha curcas l. seed were investigated. the results showed that germination rates of seeds stored at room temperature and 4°c for 1 month was significantly higher than those stored for 3 months at -20 and -80°c. mda ...

background: cutaneous leishmaniasis (cl) with diverse clinical manifestations is prevalent and remains a major public health problem in iran and its incidence has been doubled over the last decade. the present study is about the potential role of rodents in the epidemiology of cl in kharameh district in shiraz, southern iran. methods: from april 2004 to april 2005, a total of sixteen rodents we...

The influence of polyamines (PAs), putrescine (Put) and spermidine (Spd) on the efficiency gynogenesis in ovule cultures red beet (syn. beetroot) (Beta vulgaris L. vulgaris) cultivar “Czerwona Kula” breeding accessions no. 3/2010 7/2008 was investigated. effect Put process plant regeneration from gynogenetic embryos studied. response to applied PAs strongly dependent genotype. In Kula”, an incr...