Background: Diabetes mellitus, a condition of chronic hyperglycemia, represents one of the greatest concerns to modern global health. Diabetes is a serious metabolic disorders with numerous complications. It is well known that, elevation of blood glucose levels leads to structural and functional changes in various target tissues and organs. The objective of the present study was to verify if th...

Side effects of ciprofloxacin (CPFX), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. In the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm DNA damage using Aniline Blue and Acridine Orange technique, respectively. The fertility potential in male mice was also evaluated. NMRI male...

Background: To establish importance of sperm DNA assessment in resolving the unexplained infertility problem. Materials and Methods: This article is based on the careful observation of texts and papers from 2000-2014 about the DNA damage assessment as a significant parameter of sperm function test. Results: Acidic Aniline blue, Sperm Chromatin Dispersion test(SCD), Malondialdehyde concentration...

BACKGROUND Evidence supports the involvement of nitric oxide (NO) in a variety of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is e...

Sperm DNA integrity is essential for the accurate transmission of genetic information. It has a highly compact and complex structure and is capable of decondensation-features that must be present in order for a spermatozoon to be considered fertile. Any form of sperm chromatin abnormalities or DNA damage may result in male infertility. In support of this conclusion, it was reported that in-vivo...

Multiple techniques have been developed to measure the amount of sperm DNA damage in an effort to identify more objective parameters for evaluation of infertile men. We now have evidence to support that integrity of sperm DNA influences a couple's fertility and helps predict the chances of pregnancy and its successful outcome. The available tests of sperm DNA damage require additional large-sca...

YES of the dye or enzyme to the sites of damaged DNA) 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 TESTS of sperm DNA integrity have been used increasingly in the evaluation of the infertile man. These tests have been developed to further our understanding of spermatogenesis and sperm function, and to...

Background Freezing process has destructive effect on different functions of sperm. Therefore, sperm motility was reduced and apoptosis and DNA damage and also levels of reactive oxygen species (ROS) were increased. Insulin-like factor-3 (INSL3) is a member of Relaxin family and directly affects on spermatogenesis and also plays a protective role against apoptosis. Therefore, with due regard to...

side effects of ciprofloxacin (cpfx), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. in the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm dna damage using aniline blue and acridine orange technique, respectively. the fertility potential in male mice was also evaluated. nmri male...

Deoxyribonucleic acid was influenced by sperm ageing and sperm storage temperature. In this study, the effect on DNA integrity at pre-freeze and post-freeze stages was analyzed by single cell gel electrophoresis during winter and summer seasons. Traditionally, the diagnosis of male infertility depends on microscopic assessment and biochemical assays to evaluate quality of semen. These tests are...