Boar spermatozoa were prepared for intracytoplasmic sperm injection (ICSI) by two different treatments to facilitate sperm chromatin decondensation and improve fertilisation rates after ICSI in pigs: spermatozoa were either frozen and thawed without cryoprotectants, or treated with progesterone. Morphological changes of the sperm heads after the treatments were examined and then the activation ...

MODIFICATIONS IN RABBIT SPERM PLASMA MEMBRANES DURING EPIDIDYMAL PASSAGE AND AFTER EJACULATION WERE INVESTIGATED BY USED OF THREE LECTINS: concanavalin A (Con A); Ricinus communis I (RCA(I)); and wheat germ agglutinin (WGA). During sperm passage from caput to cauda epididymis, agglutination by WGA drastically decreased, and agglutination by RCA(I) slightly decreased, although agglutination by C...

Sexing and centrifugation of spermatozoa is one way to improve the quality spermatozoa. This study aimed obtain right time for sexing with Bovine Serum Albumin media on morphology X-Y in Simmental cattle. was carried out from October 2021 November at Laboratory Animal Reproduction Health Breeding Center Superior Livestock Forage, Sembawa. The design used this a completely randomized (CRD) 3 tre...

The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17°C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridg...

Introduction: Spermatozoa selection for Intracytoplasmic Sperm Injection (ICSI) is a paramount factor in the outcome of a fertility treatment cycle. Nature has perfected the selection process by using the cumulus matrix to select spermatozoa that are morphologically and genetically normal. Aim: To determine which method of semen preparation delivers the best results in terms of spermatozoa sele...

BACKGROUND Normal fertilization and ongoing pregnancy can be achieved using intracytoplasmic sperm injection (ICSI), even with severely immature spermatozoa. However, the published literature documents conflicting results as to the outcome of ICSI. METHODS Surgical extraction of spermatozoa in 111 ICSI treatment cycles performed over five years at the Assisted Conception Unit (ACU), Universit...

In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...

This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for...

Utilization of cauda epididymis spermatozoa has been carried out because these can be used in artificial insemination or vitro fertilization. The purpose this study was to obtain information on the quality which produced from Bali cattle at different ages. samples came 31 slaughterhouses. Information and identification age based structure incisors. Observations were made variables motility, con...

This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently su...