Multicellular spheroids composed of transformed cells are known to mimic the growth characteristics of tumors and to develop gradients in proliferation with increasing size. This progressive accumulation of quiescent cells is presumably an active process that occurs in response to the microenvironmental stresses that develop within the three-dimensional structure, and, yet, little is known rega...

Modern techniques of laser microsurgery of cell spheroids were used to develop a new simple reproducible model for studying repair and regeneration in vitro Nanosecond laser pulses (wavelength 355 nm, frequency 100 Hz, pulse duration 2 ns) were applied to perform a microdissection of the outer and the inner zones of human bone marrow multipotent mesenchymal stromal cells (BM MMSC) spheroids. To...

New fluorescence microscopy techniques, such as confocal or digital deconvolution microscopy, allow to easily obtain three-dimensional (3D) information from specimens. However, there are few 3D quantification tools that allow extracting information of these volumes. Therefore, the amount of information acquired by these techniques is difficult to manipulate and analyze manually. The present stu...

ABSTRACT We consider the gravitational potential of a rotating star with non-uniform density to derive orbital and epicyclic frequencies particles orbiting star. assume that is composed concentric spheroids constant density, global power-law distribution inside At lowest order approximation, we recover known result for Maclaurin spheroid maximum in radial frequency occurs at $r=\sqrt{2}ae$, ecc...

This is an investigation into the exact forms of images point charges in 2D conducting ellipses and 3D prolate spheroids. For ellipse with exterior source, we compare two previous expressions for analytic continuation inside down to focal line, location image charge. interior discover a sequence lying outside. source close surface spheroid, series solutions potential diverge region that encompa...

Purpose Axonal severing is critical to the irreversible disability that occurs over the course of multiple sclerosis (MS). Reactive oxygen species (ROS) are implicated in neurodegenerative aspects of MS: axonal spheroid formation, severing, and axoplasmic Ca elevation. However, the exact role of Ca in spheroid formation remains unclear. The mechanism of action of natural anti-oxidants such as l...

A Sessile Drop Method for 3D Cell Spheroid Culture In article number 2100972, Bangyong Sun, Gang Li, and co-workers demonstrate a sessile drop platform long-term spheroid culture. Benefitting from its open, upward, non-wettable features, the provides not only high mechanical stability easiness of medium exchange but also excellent antifouling ability oxygen availability, thus enabling robust cu...

An attractive option for tissue engineering is to use of multicellular spheroids as microtissues, particularly with stem cell spheroids. Conventional approaches of fabricating spheroids suffer from low throughput and polydispersity in size, and fail to supplement cues from extracellular matrix (ECM) for enhanced differentiation. In this study, we report the application of microfluidics-generate...

Balloon pumps are employed to assist cardiac function in cases of acute myocardial infarction, ventricular arrhythmias, cardiogenic shock, unstable angina, refractory failure, or surgery. Counterpulsation, through increasing the diastolic pressure and reducing systolic pressure, increases coronary perfusion assists heart pump more blood at each contraction. An expanding-contracting balloon, mod...

Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF) plays a crucial role in oviductal contraction and is produced by oviductal epithelial cells in cattle. Since stromal cells of many organs produce PGF, PGF production by bovine oviductal stromal cells was investigated. A...