We recently described successful molecular diagnosis of B. burgdorferi DNA by nested PCR in urine in patient-derived samples only after extraction with DNAzol (1). The aim of this study was to examine whether Borrelia burgdorferi DNA can also be detected in urine by quantitative one-step real-time PCR (Q-PCR). Q-PCR was performed after extraction with DNAzol (Molecular Research Center Inc., Cin...

An in-capillary sample preconcentration strategy based on solid phase extraction (SPE) technology coupled with capillary electrophoresis (CE) has been developed taking advantage of both techniques (SPE and CE). An in-line frit-free preconcentration device for capillary electrophoresis containing MCX beads, obtained from the corresponding Waters OASIS cartridges, was prepared. The retention of t...

Synthetic cannabinoids are a series of synthetic substances that mimic the effects natural and produce much stronger toxicity than cannabinoids, they have become most abused family new psychoactive substances. A solid-phase extraction–liquid chromatography–triple quadrupole/linear ion trap mass spectrometry method has been developed to determine 11 in rat urine. Oasis HLB cartridge was selected...

The introduction of ecological sanitation (ECOSAN) toilets in villages near Bangalore hascreated opportunities for safer sanitation and recycling of human excreta, as fertilizers, in ruraland peri-urban areas. Field experiments were conducted at the University farm on French beansand Maize as the test crops in succession for 2 consecutive years in the same field. Differenttreatment combinations...

The presence of endogenous amplification inhibitors in urine may produce false-negative results for the detection of Chlamydia trachomatis nucleic acids by tests such as PCR, ligase chain reaction (LCR), and transcription-mediated amplification (TMA). Consecutive urine specimens from 101 pregnant women and 287 nonpregnant women submitted for urinalysis were processed for C. trachomatis detectio...

Biomonitoring of lead (Pb) in blood and urine enables quantitative evaluation of human occupational and environmental exposures to Pb. State-of-the-art ICP-MS instruments can only analyze metals in laboratories, resulting in lengthy turnaround times, and they are expensive. In response to the growing need for a metal analyzer capable of on-site, real-time monitoring of trace toxic metals in ind...

Many studies suggest that mutagenic/carcinogenic chemicals in the diet, like 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), may play a role in human cancer initiation. We have developed a method to quantify PhIP metabolites in human urine and have applied it to samples from female volunteers who had eaten a meal of cooked chicken. For this analysis, urine samples (5 ml) were spiked wit...

Heroin is an illegal drug and monitoring heroin use is important in rehabilitation, pain management services, and workplace employment testing. In humans, heroin is rapidly converted to 6-acetylmorphine (6AM), which is quickly hydrolyzed to morphine. Morphine glucuronide is the major metabolite of heroin in urine (1 ). However, 6AM is a unique metabolite of heroin and is considered the definiti...

An impressively simple and precise spectrofluorimetric procedure was established and validated for ponatinib (PTB) quantitation in biological fluids such as human plasma and human urine. This method depends on examining the fluorescence characteristics of PTB in a micellar system of Cremophor RH 40 (Cr RH 40). Cr RH 40 enhanced the intrinsic fluorescence of PTB distinctly in aqueous water. The ...

An innovative method for the determination of pesticides different uses and from chemical families and/or their known metabolites in human urine was developed. The protocol includes an enzymatic hydrolysis followed by off-line solid phase extraction (SPE) step then a targeted quantitative analysis ultra-high performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass sp...