Interspecific hybridization among the three most economically important cultivated species of Cucurbita spp., Cucurbita pepo, C. moschata, and C. maxima can be made but not readily. By means of various pollination measures, different mating systems, and varying selection methods, nine advanced interspecific-bridge lines were developed, in which the crossing barrier among the species and the mal...

This study was designed to answer the question: Is H3-thymidine uptake by nuclei of the mouse seminal vesicle evidence for DNA synthesis and mitosis, or does it signify some "metabolic" function of DNA unrelated to chromosome duplication? Mice were given an intraperitoneal injection of H3-thymidine. Six hours later Feulgen squashes of the seminal vesicle epithelium were made and covered with au...

The identification and specific localization of chemical constituents within the cell nucleus have lagged considerably behind the qualitative and quantitative characterization of these classes of substances, with the possible exception of one component, the deoxyribonucleic acid (DNA). In addition to the latter, the nucleus contains ribonucleic acid1 2 (RNA), histone or basic protamine3 (BP), a...

This study was designed to answer the question: Is H3-thymidine uptake by nuclei of the mouse seminal vesicle evidence for DNA synthesis and mitosis, or does it signify some "metabolic" function of DNA unrelated to chromosome duplication? Mice were given an intraperitoneal injection of H3-thymidine. Six hours later Feulgen squashes of the seminal vesicle epithelium were made and covered with au...

ourmia melon virus (oumv) is the causal agent of mosaic disease on melon in west-azerbaijan province and probably in some other parts of iran. symptomatic and non-symptomatic samples of cultivated and non-cultivated hosts were collected from most cucurbit-planting parts of the province and surveyed using common serological methods namely: indirect-elisa, direct-elisa and lateral flow. some squa...

had an equivalent negative impact. Indeed, participants in a 2007 workshop sponsored by the National Academy of Sciences, entitled ‘Genetically Engineered Organisms, Wildlife, and Habitat’, agreed on the importance of proper comparisons. Referring to the findings of meeting participant LaReesa Wolfenbarger from University of Nebraska, a summary of the workshop said, “The conclusion Wolfenbarger...

Allium roots grown in C14-thymidine and H3-thymidine media were treated with N hydrochloric acid at 60°C. as in standard Feulgen hydrolysis. The retention of the radioactive thymidine in DNA as a function of hydrolysis time was studied autoradiographically. No significant loss of label was detected until hydrolysis was extended beyond the optimal time for Feulgen staining. The data are consiste...

We have cloned a novel transposable element from the nepY chromosome of Drosophila miranda. The size of the element, designated as TRAM, is 3.452 bp, including on both sides long terminal direct repeats (LTRs) of 372 bp, respectively. The element is flanked by a 5-bp target site duplication, ATATG. The putative primer binding site (PBS) for minus-strand priming is complementary to 18 nucleotide...

Treatment with the antitubulin vinblastine was found to disrupt the spindle system in dividing root-tip cells of common wheat, Triticum aestiuum L. Genotypes lacking the somatic association suppressor gene on 5BL, or containing the somatic-association promoter on 5BS, were found to be more sensitive to the treatment. In genetic lines carrying the somatic association suppressor, sensitivity to v...

In situ hybridization has become a standard method for localizing DNA or RNA sequences in cytological preparations. We developed two methods to extend this technique to the transmission electron microscope level using mouse satellite DNA hybridization to whole mount metaphase chromosomes as the test system. The first method devised is a direct extension of standard light microscope in situ hybr...