INTRODUCTION Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. OBJECTIVE Therefore, the goal of this study was to identify these microorganisms by both phenotyping and polymerase chain reaction (PCR) technique. ...

Here we report one of the smallest real-time polymerase chain reaction (PCR) systems to date with an approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in the form of virtual reaction chambers (VRCs) where a ≈200 nL sample is covered with mineral oil and placed on a glass cover slip. Fas...

INTRODUCTION A semi-automated magnetic capture probe-based DNA extraction and real-time PCR method (MC-PCR), allowing for a more efficient large-scale surveillance of Echinococcus multilocularis occurrence, has been developed. The test sensitivity has previously been evaluated using the sedimentation and counting technique (SCT) as a gold standard. However, as the sensitivity of the SCT is not ...

We report a competitive reverse transcriptase-PCR (RT-PCR) assay and a calibrated user-friendly RT-PCR assay (CURT-PCR) for epidermal growth factor receptor (EGFR) mRNA. A calibrator was prepared from isolated rat liver RNA, and the amount of EGFR mRNA was determined by competitive RT-PCR. In CURT-PCR, a calibration curve was developed by plotting the ratio between the amount of PCR product ori...

For diagnosis of citrus bacterial canker by PCR, an internal standard is employed to ensure the quality of the DNA extraction and that proper requisites exist for the amplification reaction. The ratio of PCR products from the internal standard and bacterial target is used to estimate the initial bacterial concentration in citrus tissues with lesions.

Enzyme immunoassay and polymerase chain reaction have become the «gold standard» for detection of biological pathogens. The method amplified – immuno-PCR allows to combine both methods into a single platform preserve their advantages achieve high sensitivity analysis. purpose this work is consider possibilities prospects using PCR-amplified pathogenic agents. Immuno-PCR makes it possible detect...

This infographic outlines evidence-based recommendations on COVID-19 reverse transcriptase PCR (RT-PCR) testing in elite sport settings, aiming to protect personal and population health, acknowledging resources expertise that are often available sport. Public health vary by country region, protocol decisions should be made consultation with relevant public authorities. An expert, multidiscip...