The unbiased sampling techniques of stereology have been developed to avoid the inaccuracies of using 'representative' sections for morphometric studies. In order to carry out a stereologically valid study, the region of interest must be fully available for sampling and its boundaries or its constituents must also be distinctly identifiable. However, in the setting of a brain bank in which only...

Stereology has been earlier defined by Wiebel (1970) to be: “a body of mathematical methods relating to three dimensional parameters defining the structure from two dimensional measurements obtainable on sections of the structure.” SHTEREOM I is a simple windows-based software for stereological estimation. In this first part, we describe the implementation of the number and volume estimation to...

Serial sectioning technique provides plenty of quantitative geometric information of the microstructure analyzed, including those unavailable from stereology with oneand two-dimensional probes. This may be why it used to be and is being continuously served as one of the most common and invaluable methods to study the size and the size distribution, the topology and the distribution of topology ...

BACKGROUND Stereology is the study of estimating geometric quantities. When successfully applied, the combination of immunohistochemistry (IHC) and stereology eliminates intra- and interobserver variability for cell type identification. METHODOLOGY/PRINCIPAL FINDINGS We propose a method to validate existing antibody based cell type markers for stereological application. Comparison was made on...

Although the human eye is excellent for pattern recognition, it often lacks the sensitivity to detect subtle changes in particle density. Because of this, quantitative evaluation may be required in some studies. A common type of quantitative assessment used for routine toxicology studies is two-dimensional histomorphometry. Although this technique can provide additional information about the ti...

the aim of the present study was stereological evaluation of testes of azoospermic animal model using busulfan in rat. three groups of male adult rats were used in this study. the first group was injected by single dose of busulfan (10 mg kg-1) and their testes were removed on day 35 post injection. the second group received double doses of busulfan with 21 days interval and their testes were r...