Suspensions of Eubacterium sp. strain 144, prepared from cells grown with 16-dehydroprogesterone, catalyzed the reduction of this steroid to 17-isoprogesterone at a very low rate. Modifications of the assay to optimize the pH (5.5) and increase the steroid solubility (10% [vol/vol] methanol) did not significantly enhance the reaction. However, growth of strain 144 in the presence of hemin was f...

In eight normals, eight anxiety neurotics and eight patients of acute schizophrenic episode, twenty-four-hour urinary excretion of each of the following was measured: (1) 17-ketosteroids (17-KS)-total, glucuronides) and sulphates, (2)17-hydroxycorticosteroids 17-KS)-total, conjugated and free, (3) Vanilamendelic acid (VMA), and (4) total indoles. In case of schizophrenics, such measurements wer...

The effects of steroid sex hormones on the established cell lines derived from human urinary bladder cancer, T24, and from human transitional cell cancer of the urinary tract, 253J, were examined using the colony formation method. Of the seven kinds of steroid hormones tested, estradiol-17 beta was intensively cytotoxic for both cells. The cytotoxic effect was depended on the dose and time of t...

Some cytochrome P450 enzymes epoxidize unsaturated substrates, but this activity has not been described for the steroid hydroxylases. Physiologic steroid substrates, however, lack carbon-carbon double bonds in the parts of the pregnane molecules where steroidogenic hydroxylations occur. Limited data on the reactivity of steroidogenic P450s toward olefinic substrates exist, and the study of occu...

A library of steroid glucuronides was prepared using the glucuronylsynthase derived from Escherichia coliβ-glucuronidase, followed by purification using solid-phase extraction. A representative range of steroid substrates were screened for synthesis on the milligram scale under optimised conditions with conversions dependent on steroid substitution and stereochemistry. Epiandrosterone (3β-hydro...

Abstract Background Nephrotic syndrome is characterized by proteinuria, hyperlipidemia, and edema. The annual incidence of nephrotic 2–7 cases/100,000 children. Methods results present study included 32 control, steroid-sensitive cases, steroid-resistant cases aged ≤ 17 years. Serum samples were handled to check serum albumin, creatinine, calcium, total cholesterol in SSNS SRNS. Pearson’s corre...

BACKGROUND/AIM periodic measurement of plasma concentrations of cortisol precursors on a clinic visit may be of limited value in patients with congenital adrenal hyperplasia because it does not reflect a patient's circadian patterns of adrenal steroid secretion. Steroid profiling in dried blood spots (DBS) may allow for more frequent and sensitive monitoring. METHODS we compared the agreement...

Adrenal cortex antibodies (ACA) were measured by immunofluorescence in 8840 adult patients with organ-specific autoimmune diseases without overt hypoadrenalism. Sixty-seven (0.8%) patients were ACA-positive, with the highest prevalence in those with premature ovarian failure (8.9%). Forty-eight ACA-positive and 20 ACA-negative individuals were enrolled into a prospective study. Antibodies to st...