Ribonuclease (RNase) Sa3 is secreted by the Gram-positive bacterium Streptomyces aureofaciens. The enzyme catalyzes the cleavage of RNA on the 3' side of guanosine residues. Here, x-ray diffraction analysis was used to determine the three-dimensional structure of two distinct crystalline forms of RNase Sa3 to a resolution of 2.0 and 1.7 A. These two structures are similar to each other as well ...

glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified andinactivated by pyridoxal 5′-phosphate (plp). the inactivation was a pseudo-first order and time-dependentreaction. complete inactivation was achieved at 0.2mm plp within 16 minutes. the type of inhibition wascompetitive with respect to glucose 6- phosphate. spectral characteristics of plp-enzyme complexcorresponde...

H. Beiderbeck, D. Risse, H. Budzikiewicz, K. Taraz Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Köln, Germany Z. Naturforsch. 54c, 1Ð5 (1999); received September 9, 1998 Pseudomonas aureofaciens, Pyoverdin, Siderophore From Pseudomonas aureofaciens a new pyoverdin was isolated and its structure was determined by various spectroscopic methods and by partial degrad...

The biological activity of Streptomyces aureofaciens MY18 and Streptomyces roseviolaceus MR13 (local isolates) as influenced by temperature,aeration, different media and carbon and nitrogen sources were investigated. It was found that biomass., growth parameters and antibiotic activity of bOth strains were highly affected by these factors. The first strain showed higher temperature range than t...

ptomyces. Thiolutin (aureothricin)9), an antibiotic produced by a new species of streptomyces, S. thioluteus, inhibits the growth of both bacteria and fungi. The writers' preliminary examination of various streptomyces indicated that S. griseus, S. aureofaciens, S. radiae, S. lavendulae, and several other strains produced substances inhibitorily effective to Candida albicans. Besides, two strai...