The objectives of this study were to induce and characterize verotoxin-encoding phages from a collection of 91 verotoxin-producing Escherichia coli (VTEC) O103:H2 strains of human and bovine origins. All the strains carried the vt1 gene, and two carried the vt2 gene as well. The phages were induced by UV irradiation and characterized by DNA restriction fragment length polymorphism (RFLP), genom...

objective: recently, phage display libraries have received enormous attention for identification and isolation of pharmaceutical molecules with diagnostic and therapeutic properties. peptide libraries are known as one of the most important and widely used types of phage display libraries. in the current study, we aimed to screen the ph.d.tm-7 phage display peptide library through biopanning for...

objective: melanocortin-4 receptor (mc4r) and agouti-related peptide (agrp) are involved in energy homeostasis in the rat. the aim of the present study was to evaluate the expression of mc4r and agrp mrnas in arcuate nucleus (arc) during long term malnutrition of female ovariectomized rats. materials and methods: ten female ovariectomized rats were divided into two equal groups (n=6) of normal ...

objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

objective(s) recent studies on human indicate that the introduction of therapeutic use of tolerogenic dendritic cell (dc) for chronic inflammatory conditions is imminent. for the purpose of defining cgrp potency in tolerogenic dc production, we investigated the phenotype and il-'2 production of dcs generated from the monocytes of rheumatoid arthritis (ra) patients in the presence of the ca...

introduction: gonadotropin inhibitory hormone (gnih) and agouti-related peptide (agrp) are orexigenic peptides expressed in the arcuate nucleus (arc) of the hypothalamus in the ewe. in addition, effects of gnih and agrp on the regulation of gonadotropin releasing hormone secretion have been shown in some mammals. the objective of the present study was to investigate the coexpression of gnih and...

in this study the bacterial growth inhibitory activity of peptide fragments produced from goat’s milk whey proteins by enzymatic hydrolysis using trypsin, ficin and a combination of both was investigated. goat’s milk whey proteins were isolated and subjected to enzymatic hydrolysis and peptides were purified by ultrafiltration followed by reverse-phase high-performance liquid chromatography (rp...

conclusions these data suggest that single bilateral microinjection of aβ (25-35) could impair memory and can be used as an ad model in wistar rats. results the results showed that aβ (25-35) significantly impaired both step-through latency and time in dark compartment in the passive avoidance task. background alzheimer's disease (ad) is the most common form of dementia that leads to neurotoxic...

collagen, which is widely distributed in pluricellular animals, is one of the most fundamental constituents of the extracellular matrix, and plays mechanically or physiologically important roles in their bodies. in this study, the biochemical and physical characteristics of pepsin-solubilized collagen from the mantle of yesso scallop (ympc), a by-product of processing, was determined. electroph...

alzheimer’s disease (ad) is a progressive neurodegenerative disorder that affects regions of the brain that control cognition, memory, language, speech and awareness to one’s physical surroundings. the pathological initiation and progression of ad is highly complex and its prevalence is on the rise. in his study, alzheimer's disease was induced with single injection of amyloid-β (aβ) pepti...