In this study, it was aimed to investigate the relationship between three SNP on BRCA1 gene and subclinical mastitis, as well milk yields during four lactation periods in Holstein breed cattle. The animal material of study consisted 151 cows reared under same care feeding conditions. Complete DNA isolated from blood samples by using phenol: chloroform: isoamyl alcohol method. Genotyping G22231T...

The objective of this research was to explore the potential association between Coxiella burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. In two separate studies, we identified an association between PCR-based detection of C. burnetii in milk and chronic subclinical mastitis in lactating dairy cows. These studies were conducted in a commercial dairy herd where there w...

The economic effect of lactational antibiotic treatment of chronic subclinical intramammary infections due to Streptococcus uberis or Streptococcus dysgalactiae was explored by means of partial budgeting. Effects at cow level and herd level were modelled, including prevention of clinical mastitis episodes and the prevention of transmission of infections. Input variables for our deterministic mo...

14 15 In this paper the implementation of a tool was considered, based on Machine Learning (ML) techniques to improve 16 the detection of subclinical mastitis in dairy herds. The analysed population included 71 dairy farms in Asturias 17 (Northern Spain) and a total of 2,407 lactating cows. Four sources of information were available: a) a questionnaire 18 survey describing facilities, milking r...

Bovine mastitis is the most important source of loss for the growing dairy industry. Streptococci, with special reference to Streptococcus agalactiae, S. dysgalactiae and S. uberis, are the predominant pathogens causing bovine mastitis. A rapid, sensitive and specific test for the detection of these pathogens needs to be developed. To accomplish this, initially 163 milk samples were collected f...

Bovine mastitis is a major disease affecting dairy cattle worldwide. The milk collected from cows with different type of inflammation, including mastitis, is characterized by an increased number of somatic cells (SCC), especially polymorphonuclear cells (PMN). It was proved that the antibacterial activity of PMN generates reactive oxygen species and nitric oxide-derived metabolites. The accumul...

Diagnosis of subclinical mastitis is of increasing importance and appropriate detection methods are needed. Both haptoglobin (Hp), an acute phase protein in cattle, as well as lactate dehydrogenase (LDH), an ubiquitous enzyme, can be successfully used to detect clinical mastitis. The present paper describes quantification of Hp and LDH in milk samples from healthy and subclinically diseased udd...

Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr) locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. a...

Milk and blood serum from clinically mastitis infected, subclinically mastitis infected and healthy Friesian cows (15 samples from each of 3 groups) were evaluated for macrominerals (sodium, potassium, calcium, magnesium and phosphorus). The milk from cows infected with subclinical mastitis revealed a significant decrease in potassium (P < 0.001) and a significant increase in sodium and phospho...

BACKGROUND AND AIM Mastitis is a serious disease of dairy animals causing great economic losses due to a reduction in milk yield as well as lowering its nutritive value. The application of somatic cell count (SCC) and alkaline phosphatase activity in the milk for diagnosis of mastitis in buffalo is not well documented. Therefore, the present study was conducted to observe the SCC and alkaline p...