DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free p...

site cleavage, the spliceosome rearranges to permit exon ligation (Umen and Guthrie, 1995b), the mRNA and lariat products are released (Company et al. Pre-mRNA splicing requires dramatic RNA rearrange-Since this pathway is conserved from yeast to mammals, ments hypothesized to be catalyzed by ATP-depen-it likely reflects fundamental requirements for intron rec-dent RNA unwindases of the DExD/H ...

In this paper, we present a switch based on coupling and resonance in thegraphene plate and rings operating at 10 THz. This structure consists of several layers ofHexagonal Boron Nitride (hBN), SiO2 and P+Si, such that graphene plates and rings areinside the hBN layer. The terahertz wave is incident from the upper part of the switchand Surface Plasmons (SPs) are excited ...

Although the N-terminal BOX-I domain of the tumor suppressor protein p53 contains the primary docking site for MDM2, previous studies demonstrated that RNA stabilizes the MDM2.p53 complex using a p53 mutant lacking the BOX-I motif. In vitro assays measuring the specific activity of MDM2 in the ligand-free and RNA-bound state identified a novel MDM2 interaction site in the core domain of p53. As...

in the present study, theoretical chemical reactivates photo isomerization in cis-retinal as a natural photo switch in vision. dft hybrid functional, b3lyp and, post-hf method, were the theoretical methods applied utilizing g09 software. 6-31g+ (d,p) basis set employed for structural optimizations, and single point computations performed using b3lyp/6-31g+(d,p). the isomers cis molecule retinal...

The tumour suppressor p53 is a tetrameric protein that is phosphorylated in its BOX-I transactivation domain by checkpoint kinase 2 (CHK2) in response to DNA damage. CHK2 cannot phosphorylate small peptide fragments of p53 containing the BOX-I motif, indicating that undefined determinants in the p53 tetramer mediate CHK2 recognition. Two peptides derived from the DNA-binding domain of p53 bind ...

SUMMARY Transcription factors IIB (TFIIB) and IIE (TFIIE) bound to RNA polymerase II have been revealed by electron crystallography in projection at 15.7 A resolution. The results lead to simple hypotheses for the roles of these factors in the initiation of transcription. TFIIB is suggested to define the distance from TATA box to transcription start site by bringing TATA DNA in contact with pol...

The pressing demands of improving energy efficiency for high performance scientific computing have motivated a large body of software-controlled hardware solutions that strategically switch hardware components to a low-power state, when the peak performance of the components is not necessary. Although OS level solutions can effectively save energy in a black-box fashion, for applications with r...

class IpAddress. • SIP messages. Socket connections receive SIP messages, which must be parsed to identify each message’s corresponding elements. The JSIP library provides several classes (SipMessage, SipUri, and SipVia, for example) that perform such parsing, but we enhanced these classes to allow parsing of strings of any IPv6 addresses included in SIP messages. • Enhanced SDP support. The or...