There is a permanent and increasing interest in large amounts of RNAs synthesized in vitro by bacteriophage T7 RNA polymerase. Especially for physical studies, mg quantities of RNA are required and have in fact been synthesized (1, 2). In order to encounter the resulting demand for T7 polymerase, we have developed a simple purification scheme for mis enzyme, using the expression system of Studi...

Bacteriophage T7 is the prototype of a group of bacterial viruses that each encode a single subunit DNA-dependent RNA polymerase (RNAP). Other members of this class include the coliphages T3 and BA14, the Salmonella phage SP6, the Pseudomonas phage gh1 and the Klebsiella phage K11 (9,16). The phage RNA polymerases have proven to be useful in a variety of applications, including large-scale RNA ...

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity reference (MHOM/GT2001/U1103). Through RNAseq-based annotation coding sequences untranslated regions, we provide primer for construct sgRNA template generation CRISPR-assisted gene deletion endogenous tagging.

We have developed an extremely sensitive technique, termed immuno-detection amplified by T7 RNA polymerase (IDAT) that is capable of monitoring proteins, lipids, and metabolites and their modifications at the single-cell level. A double-stranded oligonucleotide containing the T7 promoter is conjugated to an antibody (Ab), and then T7 RNA polymerase is used to amplify RNA from the double-strande...

An improved purification procedure for Thermotoga maritima RNA-polymerase holoenzyme was developed. The enzyme is highly active with poly dAT or T7 phage DNA as template. DNA gyrase was found to be a side product of this RNA-polymerase purification. The genes for the large subunits beta and beta' of RNA-polymerase were cloned and sequenced. The phylogenetic position of T.maritima within the bac...

The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant production. Continuous culture the most common method grow cells for In study, K12 strain of E. was as production protein. LeExp1 gene plant. Since related found very small amounts plants, it has been reproduced PCR and made workable with this method. T17 vector (...