conclusions the quite sensitive in-house taqman real time rt-pcr assay was able to detect and quantify all four main hcv genotypes prevailing around all geographical regions of iran. results the lower limit detection of this in-house hcv real-time rt-pcr was determined as 100 rna copies/ml. inter- and intra-assay coefficient of variation (cv) of this in-house hcv real-time rt-pcr ranged from 0....

A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easil...

BACKGROUND Molecular tests for diagnosis of disease, particularly cancer, are gaining increased acceptance by physicians and their patients for disease prognosis and selection of treatment options. Gene expression profiles and genetic mutations are key parameters used for the molecular characterization of tumors. A variety of methods exist for mutation analysis but the development of assays wit...

EQUINE herpesvirus type 1 (EHV-1) infection is widespread in horse populations throughout the world and produces well-documented syndromes of respiratory disease, abortion, neonatal foal death and myeloencephalopathy (van Maanen 2002). The epidemiology of EHV-1 is characterised by lifelong latent infection after primary exposure. The reactivation of a latent stage in a subclinically affected an...

A DNA computer readout approach based on real-time polymerase chain reaction (PCR) for the computation of Hamiltonian Path Problem (HPP) is the main interest in this research. Based on this methodology, real-time amplification of DNA template with TaqMan probes is performed with some modifications to realize the readout. The readout approach consists of two phases: real-time amplification in vi...