This video and article contribution gives a comprehensive description of microinjection and electroporation of mouse testis in vivo. This particular transfection technique for testicular mouse cells allows the study of unique processes in spermatogenesis. The following protocol focuses on transfection of testicular mouse cells with plasmid constructs. Specifically, we used the reporter vector p...

When conducting inter-species regression analyses, the phylogenetic relationships between the individual species need to be taken into account. In this paper, a procedure for conducting such analyses is discussed, which only requires the use of a measure of relationship between pairs of species, rather than a complete phylogeny, and which at the same time assesses the importance to be attached ...

When conducting inter-species regression analyses, the phylogenetic relationships between the individual species need to be taken into account. In this paper, a procedure for conducting such analyses is discussed, which only requires the use of a measure of relationship between pairs of species, rather than a complete phylogeny, and which at the same time assesses the importance to be attached ...

A hypothesis that many potential Translational Control Elements (TCEs) may have stable secondary structures which are conserved across closely related genomes has been investigated. The 3’-UTR sequences and PAC (Poly-Adenylation Cleavage) sites information of Human and Mouse orthologous genes have been analyzed. It has been found that orthologous genes usually have very similar 3’-UTR sequences...

The structure of the testis of the goby Glossogobius olivaceus was histologically studied. The testis was a small elongate organ and was semicircular or reverse triangle in shape in cross section. The testis was composed of two main parts; peripheral seminiferous region and central large glandular region. Seminiferous region was mainly composed of seminiferous lobules. The cyst of germ cells co...

HSP110 functions to protect cells, tissues, and organs from noxious conditions. Vasectomy induces apoptosis in the testis; however, little is known about the reason leading to this outcome. The aim of the present study was to evaluate the expression and function of HSP110 in mouse testis after vasectomy. Following bilateral vasectomy, we used fluorescent Terminal deoxynucleotidyl transferase dU...

It has been a long-lasting controversial issue as to whether or not the male genital organs, such as the testis and prostate, contain metallothioneins (MTs), a group of cysteine-rich heavy-metal-binding proteins that play a role in detoxifying heavy metals such as cadmium (Cd). Earlier studies reported that the rodent testis lacks MTs and concluded that this is why the testis is very susceptibl...

Zfy-1 and Zfy-2 are candidate genes for Tdy, the testis-determining gene in mice. We have analysed these genes in a line of XY female mice that have been shown to be mutated in Tdy. We have used Southern blot analysis to show that the Zfy genes have not undergone any major structural alterations, and have also demonstrated that both genes are transcribed normally from the mutant Y chromosome (Y...

The human histone H2B variant TSH2B is highly expressed in testis and may function in the chromatin transition during spermatogenesis. In the present study, the crystal structure of the human testis-specific nucleosome containing TSH2B was determined at 2.8 Å resolution. A local structural difference between TSH2B and canonical H2B in nucleosomes was detected around the TSH2B-specific amino-aci...

INTRODUCTION Production of antibodies against specific proteins of testis germ cells is of great significance for the investigation of processes involved in spermatogenesis, study of infertility problems and determination of the probable role of these proteins as cancer-testis antigens. Murine Testis Specific Recombinant Protein 101 (mTEX101) is a 38kDa, GPI-anchored protein which is expressed ...