1. The metabolism of [4-14C]pregnenolone in vitro by boar adrenocortical and testis tissue has been studied. 2. Boar testis tissue formed three labelled A16e steroids, 5a-androst1 6-en-3oa-ol, 5a-androst1 6-en-3,B-ol and androsta-4, 16-dien3-one. In adrenal tissue very much smaller yields of the same metabolites were obtained. 3. Both tissues produced labelledprogesterone, androst-4-ene-3,17-di...

We evaluated whether inhibiting gene expression by small interfering RNA (siRNA) can be used for an in vivo model using a germ cell-specific gene (Tex101) as a model target in mouse testis. We generated plasmid-based expression vectors of siRNA targeting the Tex101 gene and transfected them into postnatal day 10 mouse testes by in vivo electroporation. After optimizing the electroporation condi...

Testis tortion in the newborn (especially antenatal testis tortion) is observed very rarely and constitutes 10-12% of childhood testis tortions. In testis tortion, firm and painless testicular tissue is palpated on physical examination. Doppler ultrasonography is a sensitive method in the diagnosis. In cases of neonatal testis tortion, the testis can be saved with appropriate surgical explorati...

Spermatogonial stem cells provide the foundation for continued adult spermatogenesis and their manipulation can facilitate assisted reproductive technologies or the development of transgenic animals. Because the pig is an important agricultural and biomedical research animal, the development of practical application techniques to manipulate the pig Spermatogonial stem cell is needed. The abilit...

Objectives: Testicular torsion is a critical medical condition necessitating prompt diagnosis and intervention. This study aimed to explore the potential safeguarding effect of carvacrol against histological damage oxidative stress resulting from torsion/detorsion (T/D) in rat testes. Materials Methods: A total 32 adult male rats were randomly divided into four groups. The sham group did not un...

background fluoxetine (flx), a selective serotonin reuptake inhibitor, is widely used in the treatment of neurological disorders. however, its application carries the risk of adverse reactions, including reproductive toxicities. grape seed is highly regarded for its pharmacological activities, specifically its antioxidant properties. objectives this study was designed to evaluate whether hydroa...

OBJECTIVES : The pathophysiology of the testicular damage in varicocele have not been completely understood. There are several studies concerning the effects of increased seminal reactive oxygen species (ROS) and the role of nitric oxide (NO) in infertile patients with varicocele and antioxidants have been used successfully to decrease oxidative stress in testis. In this study, we determined th...